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Hematoxylin and eosin (H&E) staining visualizes histology but lacks specificity for diagnostic markers. Immunohistochemistry (IHC) staining provides protein-targeted staining but is restricted by tissue availability and antibody…

图像与视频处理 · 电气工程与系统科学 2025-07-01 Anran Liu , Xiaofei Wang , Jing Cai , Chao Li

Virtual staining offers a promising method for converting Hematoxylin and Eosin (H&E) images into Immunohistochemical (IHC) images, eliminating the need for costly chemical processes. However, existing methods often struggle to utilize…

计算机视觉与模式识别 · 计算机科学 2025-11-11 Sicheng Yang , Zhaohu Xing , Haipeng Zhou , Lei Zhu

Hematoxylin and Eosin (H&E) staining is a cornerstone of pathological analysis, offering reliable visualization of cellular morphology and tissue architecture for cancer diagnosis, subtyping, and grading. Immunohistochemistry (IHC) staining…

图像与视频处理 · 电气工程与系统科学 2025-06-23 Amit Das , Naofumi Tomita , Kyle J. Syme , Weijie Ma , Paige O'Connor , Kristin N. Corbett , Bing Ren , Xiaoying Liu , Saeed Hassanpour

Immunohistochemical (IHC) staining enables precise molecular profiling of protein expression, with over 200 clinically available antibody-based tests in modern pathology. However, comprehensive IHC analysis is frequently limited by…

计算机视觉与模式识别 · 计算机科学 2026-02-27 Fuqiang Chen , Ranran Zhang , Wanming Hu , Deboch Eyob Abera , Yue Peng , Boyun Zheng , Yiwen Sun , Jing Cai , Wenjian Qin

In addition to evaluating tumor morphology using H&E staining, immunohistochemistry is used to assess the presence of specific proteins within the tissue. However, this is a costly and labor-intensive technique, for which virtual staining,…

计算机视觉与模式识别 · 计算机科学 2025-11-25 José Teixeira , Pascal Klöckner , Diana Montezuma , Melis Erdal Cesur , João Fraga , Hugo M. Horlings , Jaime S. Cardoso , Sara P. Oliveira

Hematoxylin and eosin (H&E) staining is the clinical standard for assessing tissue morphology, but it lacks molecular-level diagnostic information. In contrast, immunohistochemistry (IHC) provides crucial insights into biomarker expression,…

图像与视频处理 · 电气工程与系统科学 2025-08-05 Jingsong Liu , Xiaofeng Deng , Han Li , Azar Kazemi , Christian Grashei , Gesa Wilkens , Xin You , Tanja Groll , Nassir Navab , Carolin Mogler , Peter J. Schüffler

Hematoxylin and eosin (H&E)-stained slides are central to cancer diagnosis and monitoring, visualizing tissue architecture and cellular morphology. However, H&E lacks the molecular specificity needed to distinguish cell states and…

图像与视频处理 · 电气工程与系统科学 2026-04-14 Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Tumor spatial heterogeneity analysis requires precise correlation between Hematoxylin and Eosin H&E morphology and immunohistochemical (IHC) biomarker expression, yet current methods suffer from spatial misalignment in consecutive sections,…

计算机视觉与模式识别 · 计算机科学 2025-09-03 Yizhe Yuan , Bingsen Xue , Bangzheng Pu , Chengxiang Wang , Cheng Jin

Virtual immunohistochemistry (IHC) aims to computationally synthesize molecular staining patterns from routine Hematoxylin and Eosin (H\&E) images, offering a cost-effective and tissue-efficient alternative to traditional physical staining.…

计算机视觉与模式识别 · 计算机科学 2026-01-23 Rongze Ma , Mengkang Lu , Zhenyu Xiang , Yongsheng Pan , Yicheng Wu , Qingjie Zeng , Yong Xia

Immunohistochemical (IHC) staining provides crucial molecular characterization of tissue samples and plays an indispensable role in the clinical examination and diagnosis of cancers. However, compared with the commonly used Hematoxylin and…

计算机视觉与模式识别 · 计算机科学 2026-01-07 Mingzhou Jiang , Jiaying Zhou , Nan Zeng , Mickael Li , Qijie Tang , Chao He , Huazhu Fu , Honghui He

Virtual immunohistochemistry (IHC) staining from hematoxylin and eosin (H&E) images can accelerate diagnostics by providing preliminary molecular insight directly from routine sections, reducing the need for repeat sectioning when tissue is…

计算机视觉与模式识别 · 计算机科学 2026-03-16 Jillur Rahman Saurav , Thuong Le Hoai Pham , Pritam Mukherjee , Paul Yi , Brent A. Orr , Jacob M. Luber

Compared to hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) not only maintains the structural features of tissue samples, but also provides high-resolution protein localization, which is essential for aiding in pathology…

计算机视觉与模式识别 · 计算机科学 2025-11-04 Yuhang Kang , Ziyu Su , Tianyang Wang , Zaibo Li , Wei Chen , Muhammad Khalid Khan Niazi

Virtual staining of histopathology images (e.g., H&E-IHC) is an emerging tool in digital pathology, enabling faster and cheaper workflows by synthesizing target stains from routinely acquired slides. Yet, the quality of virtual staining…

With the rapid development of digital pathology, virtual staining has become a key technology in multimedia medical information systems, offering new possibilities for the analysis and diagnosis of pathological images. However, existing…

计算机视觉与模式识别 · 计算机科学 2025-06-10 Hao Yang , JianYu Wu , Run Fang , Xuelian Zhao , Yuan Ji , Zhiyu Chen , Guibin He , Junceng Guo , Yang Liu , Xinhua Zeng

With the advent of digital scanners and deep learning, diagnostic operations may move from a microscope to a desktop. Hematoxylin and Eosin (H&E) staining is one of the most frequently used stains for disease analysis, diagnosis, and…

计算机视觉与模式识别 · 计算机科学 2023-08-28 Shikha Dubey , Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Histological Tertiary Lymphoid Structures (TLSs) are increasingly recognized for their correlation with the efficacy of immunotherapy in various solid tumors. Traditionally, the identification and characterization of TLSs rely on…

图像与视频处理 · 电气工程与系统科学 2024-08-27 Qiuli Wang , Yongxu Liu , Li Ma , Xianqi Wang , Wei Chen , Xiaohong Yao

Immunohistochemical (IHC) staining serves as a valuable technique for detecting specific antigens or proteins through antibody-mediated visualization. However, the IHC staining process is both time-consuming and costly. To address these…

计算机视觉与模式识别 · 计算机科学 2025-04-02 Zetong Chen , Yuzhuo Chen , Hai Zhong , Xu Qiao

Immunohistochemical (IHC) staining highlights the molecular information critical to diagnostics in tissue samples. However, compared to H&E staining, IHC staining can be much more expensive in terms of both labor and the laboratory…

计算机视觉与模式识别 · 计算机科学 2023-03-14 Fangda Li , Zhiqiang Hu , Wen Chen , Avinash Kak

Histopathological cancer diagnosis is based on visual examination of stained tissue slides. Hematoxylin and eosin (H\&E) is a standard stain routinely employed worldwide. It is easy to acquire and cost effective, but cells and tissue…

计算机视觉与模式识别 · 计算机科学 2022-07-12 Zhaoyang Xu , Xingru Huang , Carlos Fernández Moro , Béla Bozóky , Qianni Zhang

Hematoxylin and Eosin (H&E) staining is widely regarded as the standard in pathology for diagnosing diseases and tracking tumor recurrence. While H&E staining shows tissue structures, it lacks the ability to reveal specific proteins that…

图像与视频处理 · 电气工程与系统科学 2025-05-21 Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian
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