相关论文: Synchrotron radiation-based tomography of an entir…
Methods for resolving the 3D microstructure of the brain typically start by thinly slicing and staining the brain, and then imaging each individual section with visible light photons or electrons. In contrast, X-rays can be used to image…
Current approaches to in vivo imaging of the mouse central nervous system (CNS) do not offer a combination of micrometer resolution and a whole-brain field of view. To address this limitation, we introduce an approach based on synchrotron…
Holistic 3D modeling of molecularly defined brain structures is crucial for understanding complex brain functions. Using emerging tissue profiling technologies, researchers charted comprehensive atlases of mammalian brain with sub-cellular…
Machine learning techniques are being increasingly applied in medical and physical sciences across a variety of imaging modalities; however, an important issue when developing these tools is the availability of good quality training data.…
We present a laser scanning reflection-matrix microscopy combining the scanning of laser focus and the wide-field mapping of the electric field of the backscattered waves for eliminating higher-order aberrations even in the presence of…
Unprecedented visual details of biological structures are being revealed by subcellular-resolution whole-brain 3D microscopy data, enabled by recent advances in intact tissue processing and light-sheet fluorescence microscopy (LSFM). These…
X-ray computed tomography is a commonly used technique for noninvasive imaging at synchrotron facilities. Iterative tomographic reconstruction algorithms are often preferred for recovering high quality 3D volumetric images from 2D X-ray…
The ANATOMIX beamline at Synchrotron SOLEIL, operational since 2018, is dedicated to hard X-ray full-field tomography techniques. Operating in a range of photon energies from approximately 5 to 50 keV, it offers both parallel-beam…
To estimate the spatial resolution of microtomographs, a test object on the submicrometer scale was prepared by focused ion beam milling and subjected to microtomographic analysis. Since human tissues are composed of cells and extracellular…
Over the last ten years, developments in whole-brain microscopy now allow for high-resolution imaging of intact brains of small rodents such as mice. These complex images contain a wealth of information, but many neuroscience laboratories…
Whole-brain neural connectivity data are now available from viral tracing experiments, which reveal the connections between a source injection site and elsewhere in the brain. These hold the promise of revealing spatial patterns of…
Brain mapping research in most neuroanatomical laboratories relies on conventional processing techniques, which often introduce histological artifacts such as tissue tears and tissue loss. In this paper we present techniques and algorithms…
Laser-driven X-ray sources are an emerging alternative to conventional X-ray tubes and synchrotron sources. We present results on microtomographic X-ray imaging of a cancellous human bone sample using synchrotron-like betatron radiation.…
The Allen Brain Atlas (ABA) of the adult mouse consists of digitized expression profiles of thousands of genes in the mouse brain, co-registered to a common three-dimensional template (the Allen Reference Atlas). This brain-wide,…
Morphology based analysis of cell types has been an area of great interest to the neuroscience community for several decades. Recently, high resolution electron microscopy (EM) datasets of the mouse brain have opened up opportunities for…
Brightfield and fluorescent imaging of whole brain sections are funda- mental tools of research in mouse brain study. As sectioning and imaging become more efficient, there is an increasing need to automate the post-processing of sec- tions…
The design of a loop-gap-resonator RF coil optimized for ex vivo mouse brain microscopy at ultra high fields is described and its properties characterized using simulations, phantoms and experimental scans of mouse brains fixed in 10%…
Ultrasound offers a radiation-free, cost-effective solution for real-time visualization of spinal landmarks, paraspinal soft tissues and neurovascular structures, making it valuable for intraoperative guidance during spinal procedures.…
Accurate reconstruction of neuronal morphology is essential for classifying cell types and understanding brain connectivity. Recent advances in imaging and reconstruction techniques have greatly expanded the scale and quality of neuronal…
Comprehensive, synapse-resolution imaging of the brain will be crucial for understanding neuronal computations and function. In connectomics, this has been the sole purview of volume electron microscopy (EM), which entails an excruciatingly…