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We present a method for a real time visualization and automatic processing for detection and classification of untouched cancer cells in blood during stain free imaging flow cytometry using digital holographic microscopy and machine…

生物物理 · 物理学 2021-06-15 Noga Nissim , Matan Dudaie , Itay Barnea. , Natan T. Shaked

Motivation: Innovative microfluidic systems carry the promise to greatly facilitate spatio-temporal analysis of single cells under well-defined environmental conditions, allowing novel insights into population heterogeneity and opening new…

机器学习 · 计算机科学 2021-05-18 Dominik Stallmann , Jan P. Göpfert , Julian Schmitz , Alexander Grünberger , Barbara Hammer

Automated in-vitro cell detection and counting have been a key theme for artificial and intelligent biological analysis such as biopsy, drug analysis and decease diagnosis. Along with the rapid development of microfluidics and lab-on-chip…

计算机视觉与模式识别 · 计算机科学 2020-01-08 Tiancheng Xia , Richard Jiang , YongQing Fu , Nanlin Jin

Detection of blood cells in microscopic images has become a major focus of medical image analysis, playing a crucial role in gaining valuable insights into a patient's health. Manual blood cell checks for disease detection are known to be…

计算机视觉与模式识别 · 计算机科学 2025-07-28 Ahmed Endris Hasen , Yang Shangming , Chiagoziem C. Ukwuoma , Biniyam Gashaw , Abel Zenebe Yutra

Uncovering the heterogeneity of cell populations is a long-standing goal in fields ranging from antimicrobial resistance to cancer research. Emerging technology platforms such as droplet microfluidics hold the promise to decipher cellular…

图像与视频处理 · 电气工程与系统科学 2020-01-29 Vasileios Anagnostidis , Benjamin Sherlock , Jeremy Metz , Philip Mair , Florian Hollfelder , Fabrice Gielen

Label-free cell classification is advantageous for supplying pristine cells for further use or examination, yet existing techniques frequently fall short in terms of specificity and speed. In this study, we address these limitations through…

图像与视频处理 · 电气工程与系统科学 2025-02-25 Khayrul Islam , Ratul Paul , Shen Wang , Yuwen Zhao , Partho Adhikary , Qiying Li , Xiaochen Qin , Yaling Liu

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

定量方法 · 定量生物学 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant

We present a machine learning method capable of accurately detecting chromosome abnormalities that cause blood cancers directly from microscope images of the metaphase stage of cell division. The pipeline is built on a series of fine-tuned…

Label-free single-cell imaging offers a scalable, non-invasive alternative to fluorescence-based cytometry, yet inferring molecular phenotypes directly from bright-field morphology remains challenging. We present a unified Deep Learning…

计算机视觉与模式识别 · 计算机科学 2026-05-15 Saqib Nazir , Ardhendu Behera

Circulating tumor cells (CTCs) are crucial biomarkers in liquid biopsy, offering a noninvasive tool for cancer patient management. However, their identification remains particularly challenging due to their limited number and heterogeneity.…

图像与视频处理 · 电气工程与系统科学 2025-11-24 Martina Russo , Giulia Bertolini , Vera Cappelletti , Cinzia De Marco , Serena Di Cosimo , Petra Paiè , Nadia Brancati

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

定量方法 · 定量生物学 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

A unique sample independent 3D self calibration methodology is tested on a unique optical coherence tomography and multi-spectral scanning laser ophthalmoscope (OCT-SLO) hybrid system. Operators visual cognition is replaced by computer…

医学物理 · 物理学 2024-08-08 Mayank Goswami

Circulating blood cell clusters (CCCs) containing red blood cells (RBCs), white blood cells(WBCs), and platelets are significant biomarkers linked to conditions like thrombosis, infection, and inflammation. Flow cytometry, paired with…

计算机视觉与模式识别 · 计算机科学 2025-10-21 Suqiang Ma , Subhadeep Sengupta , Yao Lee , Beikang Gu , Xianyan Chen , Xianqiao Wang , Yang Liu , Mengjia Xu , Galit H. Frydman , He Li

Blood cell classification and counting are vital for the diagnosis of various blood-related diseases, such as anemia, leukemia, and thrombocytopenia. The manual process of blood cell classification and counting is time-consuming, prone to…

Liquid biopsies (eg., blood draws) offer a less invasive and non-localized alternative to tissue biopsies for monitoring the progression of metastatic breast cancer (mBCa). Immunofluoresence (IF) microscopy is a tool to image and analyze…

计算机视觉与模式识别 · 计算机科学 2024-10-07 Evan Schwab , Bharat Annaldas , Nisha Ramesh , Anna Lundberg , Vishal Shelke , Xinran Xu , Cole Gilbertson , Jiyun Byun , Ernest T. Lam

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

Immunoaffinity-based liquid biopsies of circulating tumor cells (CTCs) hold great promise for cancer management, but typically suffer from low throughput, relative complexity and post-processing limitations. Here we address these issues…

定量方法 · 定量生物学 2026-04-29 Jing Yan , Jie Wang , Robert Dallmann , Renquan Lu , Jérôme Charmet

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

定量方法 · 定量生物学 2025-11-03 Surajit Das , Pavel Zun

Accurate molecular quantification is essential for advancing research and diagnostics in fields such as infectious diseases, cancer biology, and genetic disorders. Droplet digital PCR (ddPCR) has emerged as a gold standard for achieving…

定量方法 · 定量生物学 2025-01-17 Yuanyuan Wei , Yucheng Wu , Fuyang Qu , Yao Mu , Yi-Ping Ho , Ho-Pui Ho , Wu Yuan , Mingkun Xu

Breast cancer has become a symbol of tremendous concern in the modern world, as it is one of the major causes of cancer mortality worldwide. In this regard, breast ultrasonography images are frequently utilized by doctors to diagnose breast…

图像与视频处理 · 电气工程与系统科学 2022-02-15 Muhammad Sakib Khan Inan , Fahim Irfan Alam , Rizwan Hasan
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