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Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

光学 · 物理学 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Motivation: Serial section microscopy is an established method for detailed anatomy reconstruction of biological specimen. During the last decade, high resolution electron microscopy (EM) of serial sections has become the de-facto standard…

计算机视觉与模式识别 · 计算机科学 2016-06-20 Philipp Hanslovsky , John A. Bogovic , Stephan Saalfeld

Confocal fluorescence microscopy is widely applied for the study of point-like emitters such as biomolecules, material defects, and quantum light sources. Confocal techniques offer increased optical resolution, dramatic fluorescence…

Despite advances in low-light level detection, single-photon methods such as photon correlation have rarely been used in the context of imaging. The few demonstrations, for example of sub-diffraction limited imaging utilizing quantum…

光学 · 物理学 2017-03-14 Yonatan Israel , Ron Tenne , Dan Oron , Yaron Silberberg

Automatic detection and segmentation of objects in 2D and 3D microscopy data is important for countless biomedical applications. In the natural image domain, spatial embedding-based instance segmentation methods are known to yield…

图像与视频处理 · 电气工程与系统科学 2021-04-30 Manan Lalit , Pavel Tomancak , Florian Jug

Microscopy imaging is vital in biology research and diagnosis. When imaging at the scale of cell or molecule level, mechanical drift on the axial axis can be difficult to correct. Although multi-scale networks have been developed for…

图像与视频处理 · 电气工程与系统科学 2023-05-31 Jiahe Wang , Boran Han

Contrast-Enhanced Ultra-Sound (CEUS) has become a viable method for non-invasive, dynamic visualization in medical diagnostics, yet Ultrasound Localization Microscopy (ULM) has enabled a revolutionary breakthrough by offering ten times…

计算机视觉与模式识别 · 计算机科学 2023-07-19 Christopher Hahne , Raphael Sznitman

Optical imaging plays a crucial role in advancing science and technology, enabling applications in fields ranging from biomedicine to astronomy. However, imaging through scattering media such as biological tissues, fog, or turbulent…

Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

图像与视频处理 · 电气工程与系统科学 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

Hyperspectral imaging is a powerful technology that is plagued by large dimensionality. Herein, we explore a way to combat that hindrance via non-contiguous and contiguous (simpler to realize sensor) band grouping for dimensionality…

图像与视频处理 · 电气工程与系统科学 2019-05-31 Muhammad Aminul Islam , Derek T. Anderson , John E. Ball , Nicolas H. Younan

Flexible intelligent metasurface (FIM) has emerged as a transformative technology to enhance wireless sensing by dynamically morphing its three-dimensional (3D) surface shape and electromagnetic response. Unlike conventional rigid arrays,…

信息论 · 计算机科学 2025-07-01 Zihao Teng , Jiancheng An , Lu Gan , Naofal Al-Dhahir , Zhu Han

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…

Ultrasound Localization Microscopy (ULM) is a non-invasive technique that allows for the imaging of micro-vessels in vivo, at depth and with a resolution on the order of ten microns. ULM is based on the sub-resolution localization of…

图像与视频处理 · 电气工程与系统科学 2025-10-17 Brice Rauby , Paul Xing , Jonathan Porée , Maxime Gasse , Jean Provost

Fibered confocal fluorescence microscopy (FCFM) is a new technique that can be used during a bronchoscopy to analyze the nature of the human bronchial and alveolar mucosa fluorescence microstructure. An endoscopic fibered confocal…

医学物理 · 物理学 2008-02-27 G. Bourg-Heckly , L. Thiberville , C. Vever-Bizet , B. Viellerobe

A widely tested approach to overcoming the diffraction limit in microscopy without disturbing the sample relies on substituting widefield sample illumination with a structured light beam. This gives rise to confocal, image-scanning and…

光学 · 物理学 2024-06-03 Alexander Duplinskiy , Jernej Frank , Kaden Bearne , A. I. Lvovsky

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…

Microsphere-assisted imaging emerged as a surprisingly simple way of achieving optical super-resolution imaging. In this work, we use movable PDMS thin films with embedded high-index barium titanate glass microspheres a sample scanning…

We propose a novel approach for automatic extraction (instance segmentation) of fibers from low resolution 3D X-ray computed tomography scans of short glass fiber reinforced polymers. We have designed a 3D instance segmentation architecture…

计算机视觉与模式识别 · 计算机科学 2019-01-07 Tomasz Konopczyński , Thorben Kröger , Lei Zheng , Jürgen Hesser

Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…

图像与视频处理 · 电气工程与系统科学 2020-01-31 Sourabh Bhide , Ralf Mikut , Maria Leptin , Johannes Stegmaier

Scanning Electron Microscopy (SEM) is indispensable in modern materials science, enabling high-resolution imaging across a wide range of structural, chemical, and functional investigations. However, SEM imaging remains constrained by…