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Virtual staining of histopathology images (e.g., H&E-IHC) is an emerging tool in digital pathology, enabling faster and cheaper workflows by synthesizing target stains from routinely acquired slides. Yet, the quality of virtual staining…

Hematoxylin and eosin (H&E)-stained slides are central to cancer diagnosis and monitoring, visualizing tissue architecture and cellular morphology. However, H&E lacks the molecular specificity needed to distinguish cell states and…

图像与视频处理 · 电气工程与系统科学 2026-04-14 Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Digital whole-slide images of pathological tissue samples have recently become feasible for use within routine diagnostic practice. These gigapixel sized images enable pathologists to perform reviews using computer workstations instead of…

人机交互 · 计算机科学 2016-10-14 Jesper Molin , Anna Bodén , Darren Treanor , Morten Fjeld , Claes Lundström

Lymph node metastasis is one of the most significant diagnostic indicators in breast cancer, which is traditionally observed under the microscope by pathologists. In recent years, computerized histology diagnosis has become one of the most…

计算机视觉与模式识别 · 计算机科学 2017-08-01 Huangjing Lin , Hao Chen , Qi Dou , Liansheng Wang , Jing Qin , Pheng-Ann Heng

Hyperspectral imaging is gaining attention in the field of disease diagnosis due to its ability to enhance tissue contrast, surpassing the capabilities of conventional brightfield imaging techniques. Typically, histological sections lack…

Poor performance of quantitative analysis in histopathological Whole Slide Images (WSI) has been a significant obstacle in clinical practice. Annotating large-scale WSIs manually is a demanding and time-consuming task, unlikely to yield the…

计算机视觉与模式识别 · 计算机科学 2023-08-08 Sarah Cechnicka , James Ball , Hadrien Reynaud , Callum Arthurs , Candice Roufosse , Bernhard Kainz

In the past decades, absorption modalities have emerged as powerful tools for label-free functional and structural imaging of cells and tissues. Many biomolecules present unique absorption spectra providing chromophore-specific information…

Whole-slide image analysis via the means of computational pathology often relies on processing tessellated gigapixel images with only slide-level labels available. Applying multiple instance learning-based methods or transformer models is…

计算机视觉与模式识别 · 计算机科学 2023-10-20 Joshua Butke , Noriaki Hashimoto , Ichiro Takeuchi , Hiroaki Miyoshi , Koichi Ohshima , Jun Sakuma

By 2025, holotomography (HT) has matured from a niche optical modality into a versatile platform for quantitative, label-free imaging in biomedicine. By reconstructing the three-dimensional refractive-index (RI) distribution of cells and…

生物物理 · 物理学 2026-01-07 YongKeun Park

Histological Tertiary Lymphoid Structures (TLSs) are increasingly recognized for their correlation with the efficacy of immunotherapy in various solid tumors. Traditionally, the identification and characterization of TLSs rely on…

图像与视频处理 · 电气工程与系统科学 2024-08-27 Qiuli Wang , Yongxu Liu , Li Ma , Xianqi Wang , Wei Chen , Xiaohong Yao

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…

Conventional histopathology has long been essential for disease diagnosis, relying on visual inspection of tissue sections. Immunohistochemistry aids in detecting specific biomarkers but is limited by its single-marker approach, restricting…

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

图像与视频处理 · 电气工程与系统科学 2024-08-06 Linhao Qu , Chengsheng Zhang , Guihui Li , Haiyong Zheng , Chen Peng , Wei He

Label-free optical absorption microscopy techniques have evolved as effective tools for non-invasive chemical specific structural, and functional imaging. Yet most modern label-free microscopy modalities target only a fraction of the…

Virtual histology is an emerging field in biomedicine that enables three-dimensional tissue visualization using X-ray micro-computed tomography. However, the method still lacks the specificity of conventional histology, in which parts of…

MUSE is a novel slide-free imaging technique for histological examination of tissues that can serve as an alternative to traditional histology. In order to bridge the gap between MUSE and traditional histology, we aim to convert MUSE images…

图像与视频处理 · 电气工程与系统科学 2020-08-20 Tanishq Abraham , Andrew Shaw , Daniel O'Connor , Austin Todd , Richard Levenson

Analysis of histopathology slides is a critical step for many diagnoses, and in particular in oncology where it defines the gold standard. In the case of digital histopathological analysis, highly trained pathologists must review vast…

计算机视觉与模式识别 · 计算机科学 2020-02-21 Pierre Courtiol , Eric W. Tramel , Marc Sanselme , Gilles Wainrib

It is commonly recognized that color variations caused by differences in stains is a critical issue for histopathology image analysis. Existing methods adopt color matching, stain separation, stain transfer or the combination of them to…

图像与视频处理 · 电气工程与系统科学 2022-08-09 Hai-Li Ye , Da-Han Wang

Manual counting of mitotic tumor cells in tissue sections constitutes one of the strongest prognostic markers for breast cancer. This procedure, however, is time-consuming and error-prone. We developed a method to automatically detect…