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Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…

Histopathological visualizations are a pillar of modern medicine and biological research. Surgical oncology relies exclusively on post-operative histology to determine definitive surgical success and guide adjuvant treatments. The current…

图像与视频处理 · 电气工程与系统科学 2022-04-04 Marian Boktor , Benjamin Ecclestone , Vlad Pekar , Deepak Dinakaran , John R. Mackey , Paul Fieguth , Parsin Haji Reza

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

Photon Absorption Remote Sensing (PARS) enables label-free imaging of subcellular morphology by observing biomolecule specific absorption interactions. Coupled with deep-learning, PARS produces label-free virtual Hematoxylin and Eosin (H&E)…

Accurate and fast histological staining is crucial in histopathology, impacting diagnostic precision and reliability. Traditional staining methods are time-consuming and subjective, causing delays in diagnosis. Digital pathology plays a…

图像与视频处理 · 电气工程与系统科学 2023-07-06 Marian Boktor , James E. D. Tweel , Benjamin R. Ecclestone , Jennifer Ai Ye , Paul Fieguth , Parsin Haji Reza

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

计算机视觉与模式识别 · 计算机科学 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

计算机视觉与模式识别 · 计算机科学 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

Histological staining is a vital step used to diagnose various diseases and has been used for more than a century to provide contrast to tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts.…

图像与视频处理 · 电气工程与系统科学 2020-05-08 Yijie Zhang , Kevin de Haan , Yair Rivenson , Jingxi Li , Apostolos Delis , Aydogan Ozcan

Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation…

Organ transplantation serves as the primary therapeutic strategy for end-stage organ failures. However, allograft rejection is a common complication of organ transplantation. Histological assessment is essential for the timely detection and…

We report a new lensless microscopy configuration by integrating the concepts of transverse translational ptychography and defocus multi-height phase retrieval. In this approach, we place a tilted image sensor under the specimen for…

图像与视频处理 · 电气工程与系统科学 2021-11-03 Shaowei Jiang , Chengfei Guo , Patrick Hu , Derek Hu , Pengming Song , Tianbo Wang , Zichao Bian , Zibang Zhang , Guoan Zheng

Joint analysis of multiple biomarker images and tissue morphology is important for disease diagnosis, treatment planning and drug development. It requires cross-staining comparison among Whole Slide Images (WSIs) of immuno-histochemical and…

图像与视频处理 · 电气工程与系统科学 2022-07-05 Abubakr Shafique , Morteza Babaie , Mahjabin Sajadi , Adrian Batten , Soma Skdar , H. R. Tizhoosh

Significance: Histopathological analysis of tissues is an essential tool for grading, staging, diagnosing and resecting cancers and other malignancies. Current histopathological techniques require substantial sample processing prior to…

医学物理 · 物理学 2021-07-14 Benjamin Ecclestone , Deepak Dinakaran , Parsin Haji Reza

Histopathological cancer diagnosis is based on visual examination of stained tissue slides. Hematoxylin and eosin (H\&E) is a standard stain routinely employed worldwide. It is easy to acquire and cost effective, but cells and tissue…

计算机视觉与模式识别 · 计算机科学 2022-07-12 Zhaoyang Xu , Xingru Huang , Carlos Fernández Moro , Béla Bozóky , Qianni Zhang

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…

Current pathology workflow involves staining of thin tissue slices, which otherwise would be transparent, followed by manual investigation under the microscope by a trained pathologist. While the hematoxylin and eosin (H&E) stain is…

医学物理 · 物理学 2021-05-04 Jingfang "Kelly" Zhang , Yuchen R. He , Nahil Sobh , Gabriel Popescu

Digital pathology has advanced significantly over the last decade, with Whole Slide Images (WSIs) encompassing vast amounts of data essential for accurate disease diagnosis. High-resolution WSIs are essential for precise diagnosis but…

图像与视频处理 · 电气工程与系统科学 2024-08-28 Xuan Xu , Saarthak Kapse , Prateek Prasanna

Holographic microscopy presents challenges for color reproduction due to the usage of narrow-band illumination sources, which especially impacts the imaging of stained pathology slides for clinical diagnoses. Here, an accurate color…

We present a rapid, large-field bimodal imaging platform that integrates conventional brightfield microscopy with a lensless IR imaging scanner, enabling whole-slide IR image stack acquisition in minutes. Using a dedicated deep learning…

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