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In this work, we describe a method for large-scale 3D cell-tracking through a segmentation selection approach. The proposed method is effective at tracking cells across large microscopy datasets on two fronts: (i) It can solve problems…

计算机视觉与模式识别 · 计算机科学 2024-04-15 Jordão Bragantini , Merlin Lange , Loïc Royer

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

计算机视觉与模式识别 · 计算机科学 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

计算机视觉与模式识别 · 计算机科学 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

图像与视频处理 · 电气工程与系统科学 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object tracking methods. In recent years, several existing and new applications have…

图像与视频处理 · 电气工程与系统科学 2025-02-03 Gergely Szabó , Paolo Bonaiuti , Andrea Ciliberto , András Horváth

This paper introduces a novel framework for the automated tracking of cells, with a particular focus on the challenging situation of phase contrast microscopic videos. Our framework is based on a topology preserving variational segmentation…

定量方法 · 定量生物学 2012-03-28 Michael Moeller , Martin Burger , Peter Dieterich , Albrecht Schwab

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

图像与视频处理 · 电气工程与系统科学 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Studying cell morphology changes in time is critical to understanding cell migration mechanisms. In this work, we present a deep learning-based workflow to segment cancer cells embedded in 3D collagen matrices and imaged with phase-contrast…

计算机视觉与模式识别 · 计算机科学 2021-12-17 Estibaliz Gómez-de-Mariscal , Hasini Jayatilaka , Özgün Çiçek , Thomas Brox , Denis Wirtz , Arrate Muñoz-Barrutia

Tracking cells and detecting mitotic events in time-lapse microscopy image sequences is a crucial task in biomedical research. However, it remains highly challenging due to dividing objects, low signal-tonoise ratios, indistinct boundaries,…

计算机视觉与模式识别 · 计算机科学 2025-09-15 Zhu Chen , Mert Edgü , Er Jin , Johannes Stegmaier

Tracking many cells in time-lapse 3D image sequences is an important challenging task of bioimage informatics. Motivated by a study of brain-wide 4D imaging of neural activity in C. elegans, we present a new method of multi-cell tracking.…

计算机视觉与模式识别 · 计算机科学 2018-01-18 Osamu Hirose , Shotaro Kawaguchi , Terumasa Tokunaga , Yu Toyoshima , Takayuki Teramoto , Sayuri Kuge , Takeshi Ishihara , Yuichi Iino , Ryo Yoshida

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

计算机视觉与模式识别 · 计算机科学 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Cell boundary information is crucial for analyzing cell behaviors from time-lapse microscopy videos. Existing supervised cell segmentation tools, such as ImageJ, require tuning various parameters and rely on restrictive assumptions about…

应用统计 · 统计学 2026-01-27 Laura Baracaldo , Blythe King , Haoran Yan , Yizi Lin , Nina Miolane , Mengyang Gu

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

图像与视频处理 · 电气工程与系统科学 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

This paper presents a method for time-lapse 3D cell analysis. Specifically, we consider the problem of accurately localizing and quantitatively analyzing sub-cellular features, and for tracking individual cells from time-lapse 3D confocal…

计算机视觉与模式识别 · 计算机科学 2022-08-18 Jiaxiang Jiang , Amil Khan , S. Shailja , Samuel A. Belteton , Michael Goebel , Daniel B. Szymanski , B. S. Manjunath

State-of-the-art object detection and segmentation methods for microscopy images rely on supervised machine learning, which requires laborious manual annotation of training data. Here we present a self-supervised method based on time arrow…

计算机视觉与模式识别 · 计算机科学 2023-07-27 Benjamin Gallusser , Max Stieber , Martin Weigert

Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…

计算机视觉与模式识别 · 计算机科学 2017-05-10 Saad Ullah Akram , Juho Kannala , Lauri Eklund , Janne Heikkilä

Cell segmentation and tracking in microscopy images are of great significance to new discoveries in biology and medicine. In this study, we propose a novel approach to combine cell segmentation and cell tracking into a unified end-to-end…

计算机视觉与模式识别 · 计算机科学 2021-02-23 Yuqian Chen , Yang Song , Chaoyi Zhang , Fan Zhang , Lauren O'Donnell , Wojciech Chrzanowski , Weidong Cai

We introduce a method for automated temporal segmentation of human motion data into distinct actions and compositing motion primitives based on self-similar structures in the motion sequence. We use neighbourhood graphs for the partitioning…

计算机视觉与模式识别 · 计算机科学 2021-12-07 Björn Krüger , Anna Vögele , Tobias Willig , Angela Yao , Reinhard Klein , Andreas Weber

Automatic cell tracking in dense environments is plagued by inaccurate correspondences and misidentification of parent-offspring relationships. In this paper, we introduce a novel cell tracking algorithm named DenseTrack, which integrates…

图像与视频处理 · 电气工程与系统科学 2024-07-08 Tanjin Taher Toma , Yibo Wang , Andreas Gahlmann , Scott T. Acton

Cell tracking enables data extraction from time-lapse "cell movies" and promotes modeling biological processes at the single-cell level. We introduce a new fully automated computational strategy to track accurately cells across frames in…

计算机视觉与模式识别 · 计算机科学 2017-06-23 Athanasios D. Balomenos , Panagiotis Tsakanikas , Elias S. Manolakos
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