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The reaction-diffusion master equation (RDME) is a standard modelling approach for understanding stochastic and spatial chemical kinetics. An inherent assumption is that molecules are point-like. Here we introduce the crowded…

统计力学 · 物理学 2016-03-23 Claudia Cianci , Stephen Smith , Ramon Grima

Brownian dynamics simulations are an increasingly popular tool for understanding spatially-distributed biochemical reaction systems. Recent improvements in our understanding of the cellular environment show that volume exclusion effects are…

化学物理 · 物理学 2017-09-13 Stephen Smith , Ramon Grima

We present a multiscale approach to model diffusion in a crowded environment and its effect on the reaction rates. Diffusion in biological systems is often modeled by a discrete space jump process in order to capture the inherent noise of…

亚细胞过程 · 定量生物学 2016-03-18 Lina Meinecke

The stochastic nature of chemical reactions involving randomly fluctuating population sizes has lead to a growing research interest in discrete-state stochastic models and their analysis. A widely-used approach is the description of the…

定量方法 · 定量生物学 2015-10-01 Alexander Andreychenko , Luca Bortolussi , Ramon Grima , Philipp Thomas , Verena Wolf

The activity of biological cells is primarily based on chemical reactions and typically modeled as a reaction-diffusion system. Cells are, however, highly crowded with macromolecules, including a variety of molecular machines such as…

生物物理 · 物理学 2018-11-02 Yuichi Togashi

Mathematical models are important tools to study the excluded volume effects on reaction-diffusion systems, which are known to play an important role inside living cells. Detailed microscopic simulations with off-lattice Brownian dynamics…

定量方法 · 定量生物学 2016-04-25 Lina Meinecke , Markus Eriksson

It is increasingly realized that taking stochastic effects into account is important in order to study biological cells. However, the corresponding mathematical formulation, the chemical master equation (CME), suffers from the curse of…

数值分析 · 数学 2023-09-18 Lukas Einkemmer , Julian Mangott , Martina Prugger

While experiments and computer simulations to study biological phenomena are usually performed in diluted in vitro conditions, such phenomena happen inside the cell, an environment densely packed with diverse macromolecules. Here, we revise…

生物大分子 · 定量生物学 2026-05-22 Apoorva Mathur , Vanessa Regina Miranda , Ariane Nunes-Alves

Investigation of protein self-assembly processes is important for the understanding of the growth processes of functional proteins as well as disease-causing amyloids. Inside cells, intrinsic molecular fluctuations are so high that they…

生物物理 · 物理学 2021-02-03 John Bridstrup , John S Schreck , Jesse L Jorgenson , Jian-Min Yuan

Biomolecular condensates are essential for cellular organization and result from phase separation in systems far from thermodynamic equilibrium. Among various models, chemically active droplets play a significant role, consisting of…

软凝聚态物质 · 物理学 2025-05-19 Jacques Fries , Roxanne Berthin , Chengjie Luo , Marie Jardat , David Zwicker , Vincent Dahirel , Pierre Illien

While ordinary differential equations (ODEs) form the conceptual framework for modelling many cellular processes, specific situations demand stochastic models to capture the influence of noise. The most common formulation of stochastic…

亚细胞过程 · 定量生物学 2009-04-02 Mukhtar Ullah , Olaf Wolkenhauer

Approximate Bayesian Computation is widely used to infer the parameters of discrete-state continuous-time Markov networks. In this work, we focus on models that are governed by the Chemical Master Equation (the CME). Whilst originally…

定量方法 · 定量生物学 2020-01-10 Christopher Lester

We investigate, via Brownian dynamics simulations, the reaction dynamics of a simple, non-linear chemical network (the Willamowski-Rossler network) under spatial confinement and crowding conditions. Our results show that the presence of…

生物物理 · 物理学 2016-10-18 Giovanni Bellesia , Benjamin B. Bales

Penalized likelihood and quasi-likelihood methods dominate inference in high-dimensional linear mixed-effects models. Sampling-based Bayesian inference is less explored due to the computational bottlenecks introduced by the random effects…

统计方法学 · 统计学 2025-07-24 Sreya Sarkar , Kshitij Khare , Sanvesh Srivastava

Intrinsic noise, the stochastic cell-to-cell fluctuations in mRNAs and proteins, has been observed and proved to play important roles in cellular systems. Due to the recent development in single-cell-level measurement technology, the…

统计方法学 · 统计学 2017-11-15 Chao Du , Wing Hong Wong

The stochastic dynamics of biochemical networks are usually modelled with the chemical master equation (CME). The stationary distributions of CMEs are seldom solvable analytically, and numerical methods typically produce estimates with…

概率论 · 数学 2019-10-30 Juan Kuntz , Philipp Thomas , Guy-Bart Stan , Mauricio Barahona

In synaptic molecular communication, the activation of postsynaptic receptors by neurotransmitters (NTs) is governed by a stochastic reaction-diffusion process and, hence, inherently random. It is currently not fully understood how this…

新兴技术 · 计算机科学 2023-01-18 Sebastian Lotter , Maximilian Schäfer , Robert Schober

A statistical mechanical theory is presented to predict the effects of macromolecular crowding on protein association equilibria, accounting for both excluded volume and attractive interactions between proteins and crowding molecules.…

软凝聚态物质 · 物理学 2015-06-11 Young C. Kim , Jeetain Mittal

The probability distribution describing the state of a Stochastic Reaction Network evolves according to the Chemical Master Equation (CME). It is common to estimated its solution using Monte Carlo methods such as the Stochastic Simulation…

定量方法 · 定量生物学 2015-06-18 Benjamin Hepp , Ankit Gupta , Mustafa Khammash

The cytoplasm of a living cell is crowded with several macromolecules of different shapes and sizes. Molecular diffusion in such a medium becomes anomalous due to the presence of macromolecules and diffusivity is expected to decrease with…

统计力学 · 物理学 2009-11-13 Manish Agrawal , S. B. Santra , Rajat Anand , Rajaram Swaminathan
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