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Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

生物物理 · 物理学 2016-08-04 Partha Pratim Mondal

In this paper, we consider the problem in defocus image deblurring. Previous classical methods follow two-steps approaches, i.e., first defocus map estimation and then the non-blind deblurring. In the era of deep learning, some researchers…

计算机视觉与模式识别 · 计算机科学 2022-07-08 Qian Ye , Masanori Suganuma , Takayuki Okatani

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

光学 · 物理学 2024-03-26 Tingting Wu , Matthew D. Lew

This work presents new experimental results on low-temperature (LT) characterization of local rf properties of passive superconducting (SC) microwave devices using a novel Laser Scanning Microscope (LSM). In this technique, a modulated…

超导电性 · 物理学 2016-11-18 A. P. Zhuravel , A. V. Ustinov , D. Abraimov , Steven M. Anlage

Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…

应用物理 · 物理学 2020-05-21 Miao-Hsuan Chien , Silvan Schmid

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

光学 · 物理学 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Existing super-resolution microscopy is often constrained by inherent trade-offs between resolution, acquisition speed, phototoxicity, and hardware complexity. Computational post-processing approaches offer a promising alternative, but they…

A new technique based on superposition of two speckle patterns is proposed and demonstrated for controlled modulation of the spatial polarization distribution of the resultant speckle. It is demonstrated both theoretically and…

光学 · 物理学 2021-11-15 Abhijit Roy , Rakesh K. Singh , Maruthi M. Brundavanam

Diffuse optical imaging (DOI) offers valuable insights into scattering mediums, but the quest for high-resolution imaging often requires dense sampling strategies, leading to higher imaging errors and lengthy acquisition times. This work…

光学 · 物理学 2025-04-07 Ben Wiesel , Shlomi Arnon

Cells sense external concentrations and, via biochemical signaling, respond by regulating the expression of target proteins. Both in signaling networks and gene regulation there are two main mechanisms by which the concentration can be…

亚细胞过程 · 定量生物学 2017-04-12 Gabriele Micali , Gerardo Aquino , David M. Richards , Robert G. Endres

We propose an enhanced spatial modulation (SM)-based scheme for indoor visible light communication systems. This scheme enhances the achievable throughput of conventional SM schemes by transmitting higher order complex modulation symbol,…

信号处理 · 电气工程与系统科学 2022-01-21 Shimaa Naser , Lina Bariah , Sami Muhaidat , Mahmoud Al-Qutayri , Paschalis C. Sofotasios

Time synchronization and phase shaping of single photons both play fundamental roles in quantum information applications that rely on multi-photon quantum interference. Phase shaping typically requires separate modulators with extra…

量子物理 · 物理学 2021-09-14 Keyu Su , Yunfei Wang , Shanchao Zhang , Zhuoping Kong , Yi Zhong , Jianfeng Li , Hui Yan , Shi-Liang Zhu

Spatial Fluorescence Cross Correlation Spectroscopy is a rarely investigated version of Fluorescence Correlation Spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported…

光学 · 物理学 2008-06-13 Yoann Blancquaert , Jie Gao , Jacques Derouard , Antoine Delon

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

We consider a microscopy setting where quantum light is used for illumination. Spontaneous parametric down conversion (SPDC) is used as a source of a heralded single photon, which is quantum light prepared in a Fock state. We present…

量子物理 · 物理学 2023-06-08 Maria Gieysztor , Joshua Nepinak , Christopher J. Pugh , Piotr Kolenderski

We investigate experimentally the nonlocal phase modulation of multiple-frequency-mode, continuous-variable entangled twin beams. We use a pair of electro-optical phase modulators to modulate the entangled probe and conjugate light beams…

Dedicated systems are fundamental for neuroscience experimental protocols that require timing determinism and synchronous stimuli generation. We developed a data acquisition and stimuli generator system for neuroscience research, optimized…

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

图像与视频处理 · 电气工程与系统科学 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

We implement a super-localization method in the time domain that allows the observation of the external motion of soliton molecules in a fiber ring cavity laser with unprecedented accuracy. In particular, we demonstrate the synchronization…

斑图形成与孤子 · 物理学 2022-06-08 Saïd Hamdi , Aurélien Coillet , Benoit Cluzel , Philippe Grelu , Pierre Colman

Speckled illumination enhances widefield fluorescence microscopy by enabling optical sectioning and super resolution. In random illumination microscopy, sequences of speckled illumination patterns are used to excite fluorescent samples and…