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Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with…

定量方法 · 定量生物学 2024-12-13 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently…

生物物理 · 物理学 2017-05-02 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically…

光学 · 物理学 2017-05-11 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

图像与视频处理 · 电气工程与系统科学 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

图像与视频处理 · 电气工程与系统科学 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

Single Pixel (SP) imaging is now a reality in many applications, e.g., biomedical ultrathin endoscope and fluorescent spectroscopy. In this context, many schemes exist to improve the light throughput of these device, e.g., using structured…

计算机视觉与模式识别 · 计算机科学 2019-02-19 Amirafshar Moshtaghpour , José M. Bioucas-Dias , Laurent Jacques

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

仪器与探测器 · 物理学 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Digital holographic microscopy based on Gabor in-line holography is a well-known method to reconstruct both the amplitude and phase of small objects. To reconstruct the image of an object from its hologram, obtained under illumination by…

光学 · 物理学 2009-11-04 S. G. Podorov , A. I. Bishop , D. M. Paganin , K. M. Pavlov

Single-shot and incoherent imaging are two important properties for digital holography technique to extend its application range. In this paper, a simple and compact in-line Fresnel incoherent digital holographic recording system with…

光学 · 物理学 2018-11-19 Dong Liang , Qiu Zhang , Jun Liu

Fourier single-pixel imaging (FSI) has proven capable of reconstructing high-quality two-dimensional and three-dimensional images. The utilization of the sparsity of natural images in Fourier domain allows high-resolution images to be…

计算机视觉与模式识别 · 计算机科学 2016-12-12 Zibang Zhang , Xueying Wang , Jingang Zhong

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

光学 · 物理学 2025-01-20 Jose Angel Picazo-Bueno , Maciej Trusiak , Javier Garcia , Vicente Mico

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

光学 · 物理学 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

光学 · 物理学 2022-03-09 James D. Manton

This paper introduces a single-pixel HyperSpectral (HS) imaging framework based on Fourier Transform Interferometry (FTI). By combining a space-time coding of the light illumination with partial interferometric observations of a collimated…

计算机视觉与模式识别 · 计算机科学 2018-09-05 Amirafshar Moshtaghpour , José M. Bioucas-Dias , Laurent Jacques

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

光学 · 物理学 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

光学 · 物理学 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

We propose a real-time digital holographic microscopy, that enables simultaneous multiple reconstructed images with arbitrary resolution, depth and positions, using Shifted-Fresnel diffraction instead of Fresnel diffraction. In this system,…

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

光学 · 物理学 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi
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