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We investigate theoretically the behavior of proteins as well as other large macromolecules which are incorporated into amphiphilic monolayers at the air-water interface. We assume the monolayer to be in the coexistence region of the…

软凝聚态物质 · 物理学 2007-05-23 R. R. Netz , D. Andelman , H. Orland

In the cell, protein complexes form relying on specific interactions between their monomers. Excluded volume effects due to molecular crowding would lead to correlations between molecules even without specific interactions. What is the…

软凝聚态物质 · 物理学 2015-05-13 Wei Wang , Wei-Xin Xu , Y. Levy , E. Trizac , P. G. Wolynes

Phase separation is the thermodynamic process that explains how droplets form in multicomponent fluids. These droplets can provide controlled compartments to localize chemical reactions, and reactions can also affect the droplets' dynamics.…

软凝聚态物质 · 物理学 2022-09-02 David Zwicker

We draw an analogy between droplet formation in dilute particle and polymer systems. Our arguments are based on finite-size scaling results from studies of a two-dimensional lattice gas to three-dimensional bead-spring polymers. To set the…

统计力学 · 物理学 2018-03-14 Wolfhard Janke , Johannes Zierenberg

The cellular cytoplasm is organized into compartments. Phase separation is a simple manner to create membrane-less compartments in order to confine and localize particles like proteins. In many cases these particles are bound to fluctuating…

Proliferation and motility are ubiquitous drivers of activity in biological systems. Here, we study a dense binary mixture of motile and proliferating particles with exclusively repulsive interactions, where homeostasis in the proliferating…

软凝聚态物质 · 物理学 2025-06-11 Lukas Hupe , Joanna M. Materska , David Zwicker , Ramin Golestanian , Bartlomiej Waclaw , Philip Bittihn

Fluorescence microscopy reveals that the contents of many (membrane-free) nuclear "bodies" exchange rapidly with the soluble pool whilst the underlying structure persists; such observations await a satisfactory biophysical explanation. To…

生物物理 · 物理学 2017-04-26 C. A. Brackley , B. Liebchen , D. Michieletto , F. Mouvet , P. R. Cook , D. Marenduzzo

Biomolecular condensates are liquid- or gel-like droplets of proteins and nucleic acids formed at least in part through liquid-liquid phase separation. Condensates enable diverse functions of cells and the pathogens that infect them,…

Biomolecular condensates are small droplets forming spontaneously in biological cells via phase separation. They play a role in many cellular processes, but it is unclear how cells control them. Cellular regulation often relies on…

软凝聚态物质 · 物理学 2024-03-15 Jan Kirschbaum , David Zwicker

Biological cells utilize membranes and liquid-like droplets, known as biomolecular condensates, to structure their interior. The interaction of droplets and membranes, despite being involved in several key biological processes, is so far…

软凝聚态物质 · 物理学 2024-02-21 Marcel Mokbel , Dominic Mokbel , Susanne Liese , Christoph A. Weber , Sebastian Aland

A microscopic model is proposed for the interactions between sickle hemoglobin molecules based on information from the protein data bank. A Monte Carlo simulation of a simplified two patch model is carried out, with the goal of…

软凝聚态物质 · 物理学 2009-11-11 Andrey Shiryayev , Xiaofei Li , James D. Gunton

Motivated by the idea that intrinsically disordered proteins (IDPs) condense into liquid-like droplets within cells, we carry out Monte Carlo simulations of a polymer lattice model to study the relationship between charge patterning and…

软凝聚态物质 · 物理学 2019-12-11 Nicholas A. S. Robichaud , Ivan Saika-Voivod , Stefan Wallin

The effect of interactions on dynamics of coupled motor proteins is investigated theoretically. A simple stochastic discrete model, that allows to calculate explicitly the dynamic properties of the system, is developed. It is shown that…

软凝聚态物质 · 物理学 2009-11-11 Evgeny B. Stukalin , Anatoly B. Kolomeisky

A simple model of globular proteins which incorporates anisotropic attractions is proposed. It is closely related to models used to model simple hydrogen-bonding molecules such as water. Theories for both the fluid and solid phases are…

软凝聚态物质 · 物理学 2009-10-31 Richard P. Sear

The concept of the surface of a protein in solution, as well of the interface between protein and 'bulk solution', is introduced. The experimental technique of small angle X-ray and neutron scattering is introduced and described briefly.…

软凝聚态物质 · 物理学 2016-03-09 R. Tscheliessnig , L. Pusztai

Biomolecular condensates self-assemble when proteins and nucleic acids spontaneously demix to form droplets within the crowded intracellular milieu. This simple mechanism underlies the formation of a wide variety of membraneless…

统计力学 · 物理学 2021-06-30 William M. Jacobs

The dilute fluid phase of model globular proteins is studied. The model possesses a fluid-fluid transition buried within the fluid-crystal coexistence region, as do some globular proteins. If this fluid-fluid transition is not buried deep…

软凝聚态物质 · 物理学 2007-05-23 Richard P. Sear

Phase separation has emerged as an essential concept for the spatial organization inside biological cells. However, despite the clear relevance to virtually all physiological functions, we understand surprisingly little about what phases…

生物物理 · 物理学 2022-08-10 David Zwicker , Liedewij Laan

Polymer models are used to describe chromatin, which can be folded at different spatial scales by binding molecules. By folding, chromatin generates loops of various sizes. We present here a randomly cross-linked (RCL) polymer model, where…

软凝聚态物质 · 物理学 2017-05-12 Ofir Shukron , David Holcman

Liquid-liquid phase separation is the mechanism underlying the formation of biomolecular condensates. Disordered protein regions often drive phase separation, but molecular interactions of disordered protein regions are not well understood,…

生物大分子 · 定量生物学 2020-08-10 Wade Borcherds , Anne Bremer , Madeleine B. Borgia , Tanja Mittag