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Despite fluorescent cell-labelling being widely employed in biomedical studies, some of its drawbacks are inevitable, with unsuitable fluorescent probes or probes inducing a functional change being the main limitations. Consequently, the…

Advancements in digital imaging technologies have sparked increased interest in using multiplexed immunofluorescence (mIF) images to visualise and identify the interactions between specific immunophenotypes with the tumour microenvironment…

图像与视频处理 · 电气工程与系统科学 2024-07-01 Piumi Sandarenu , Julia Chen , Iveta Slapetova , Lois Browne , Peter H. Graham , Alexander Swarbrick , Ewan K. A. Millar , Yang Song , Erik Meijering

Cell detection and cell type classification from biomedical images play an important role for high-throughput imaging and various clinical application. While classification of single cell sample can be performed with standard computer…

图像与视频处理 · 电气工程与系统科学 2019-12-17 Wei Qiu , Jiaming Guo , Xiang Li , Mengjia Xu , Mo Zhang , Ning Guo , Quanzheng Li

Resistive Pulse Sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells,…

生物物理 · 物理学 2020-04-01 Antoine Riaud , Anh L. P. Thai , Wei Wang , Valerie Taly

Conventional histopathology has long been essential for disease diagnosis, relying on visual inspection of tissue sections. Immunohistochemistry aids in detecting specific biomarkers but is limited by its single-marker approach, restricting…

Inspection of tissues using a light microscope is the primary method of diagnosing many diseases, notably cancer. Highly multiplexed tissue imaging builds on this foundation, enabling the collection of up to 60 channels of molecular…

Multiplexed immunofluorescence microscopy captures detailed measurements of spatially resolved, multiple biomarkers simultaneously, revealing tissue composition and cellular interactions in situ among single cells. The growing scale and…

软件工程 · 计算机科学 2025-06-03 Fang Liu , Rui He , Andrei Bombin , Ahmad B. Abdallah , Omar Eldaghar , Tommy R. Sheeley , Sam E. Ying , George Zaki

Label-free cell classification is advantageous for supplying pristine cells for further use or examination, yet existing techniques frequently fall short in terms of specificity and speed. In this study, we address these limitations through…

图像与视频处理 · 电气工程与系统科学 2025-02-25 Khayrul Islam , Ratul Paul , Shen Wang , Yuwen Zhao , Partho Adhikary , Qiying Li , Xiaochen Qin , Yaling Liu

We present a method for automatic cell classification in tissue samples using an automated training set from multiplexed immunofluorescence images. The method utilizes multiple markers stained in situ on a single tissue section on a robust…

图像与视频处理 · 电气工程与系统科学 2020-07-21 Alberto Santamaria-Pang , Anup Sood , Dan Meyer , Aritra Chowdhury , Fiona Ginty

Automated segmentation of multiple sclerosis (MS) lesions using multicontrast magnetic resonance (MR) images improves efficiency and reproducibility compared to manual delineation, with deep learning (DL) methods achieving state-of-the-art…

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…

光学 · 物理学 2023-04-26 Chungha Lee , Herve Hugonnet , Juyeon Park , Mahn Jae Lee , Weisun Park , Yongkeun Park

Cataloging the neuronal cell types that comprise circuitry of individual brain regions is a major goal of modern neuroscience and the BRAIN initiative. Single-cell RNA sequencing can now be used to measure the gene expression profiles of…

Multimodal single-cell technologies enable the simultaneous collection of diverse data types from individual cells, enhancing our understanding of cellular states. However, the integration of these datatypes and modeling the…

机器学习 · 计算机科学 2023-11-22 Bhavya Mehta , Nirmit Deliwala , Madhav Chandane

Recent advancements in single-cell genomics necessitate precision in gene panel selection to interpret complex biological data effectively. Those methods aim to streamline the analysis of scRNA-seq data by focusing on the most informative…

人工智能 · 计算机科学 2024-06-12 Weiliang Zhang , Zhen Meng , Dongjie Wang , Min Wu , Kunpeng Liu , Yuanchun Zhou , Meng Xiao

Quantitative analysis of cell nuclei in microscopic images is an essential yet challenging source of biological and pathological information. The major challenge is accurate detection and segmentation of densely packed nuclei in images…

定量方法 · 定量生物学 2019-11-14 Linqing Feng , Jun Ho Song , Jiwon Kim , Soomin Jeong , Jin Sung Park , Jinhyun Kim

We propose a general framework for a collaborative machine learning system to assist bioscience researchers with the task of labeling specific cell identities from microscopic still or video imaging. The distinguishing features of this…

定量方法 · 定量生物学 2019-03-25 Greg Bubnis , Steven Ban , Matthew D. DiFranco , Saul Kato

We describe a new multiresolution "nested encoder-decoder" convolutional network architecture and use it to annotate morphological patterns in reflectance confocal microscopy (RCM) images of human skin for aiding cancer diagnosis. Skin…

计算机视觉与模式识别 · 计算机科学 2018-08-24 Alican Bozkurt , Kivanc Kose , Christi Alessi-Fox , Melissa Gill , Dana H. Brooks , Jennifer G. Dy , Milind Rajadhyaksha

Renal Cell Carcinoma is typically asymptomatic at the early stages for many patients. This leads to a late diagnosis of the tumor, where the curability likelihood is lower, and makes the mortality rate of Renal Cell Carcinoma high, with…

计算机视觉与模式识别 · 计算机科学 2025-12-17 Mohamad Mohamad , Francesco Ponzio , Santa Di Cataldo , Damien Ambrosetti , Xavier Descombes

Unsupervised cell type identification is crucial for uncovering and characterizing heterogeneous populations in single cell omics studies. Although a range of clustering methods have been developed, most focus exclusively on intrinsic…

人工智能 · 计算机科学 2025-12-12 Liang Peng , Haopeng Liu , Yixuan Ye , Cheng Liu , Wenjun Shen , Si Wu , Hau-San Wong

Malignant lymphoma subtype classification directly impacts treatment strategies and patient outcomes, necessitating classification models that achieve both high accuracy and sufficient explainability. This study proposes a novel explainable…

计算机视觉与模式识别 · 计算机科学 2025-03-04 Daiki Nishiyama , Hiroaki Miyoshi , Noriaki Hashimoto , Koichi Ohshima , Hidekata Hontani , Ichiro Takeuchi , Jun Sakuma
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