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Photoacoustic microscopy holds the potential to measure biomarkers' structural and functional status without labels, which significantly aids in comprehending pathophysiological conditions in biomedical research. However, conventional…

图像与视频处理 · 电气工程与系统科学 2025-06-05 Wangting Zhou , Jiangshan He , Tong Cai , Lin Wang , Zhen Yuan , Xunbin Wei , Xueli Chen

Photoacoustic microscopy is becoming an important tool for the biomedical research. It has been widely used in biological researches, such as structural imaging of vasculature, brain structural and functional imaging, and tumor detection.…

图像与视频处理 · 电气工程与系统科学 2020-09-09 Xianlin Song , Jianshuang Wei , Lingfang Song

We demonstrate Optical-Resolution Photoacoustic Microscopy (OR-PAM), where the optical field is focused and scanned using Digital Phase Conjugation (DPC) through a multimode fiber. The focus is scanned across the field of view using digital…

Photoacoustic microscopy (PAM) is a promising imaging modality because it is able to reveal optical absorption contrast in high resolution on the order of a micrometer. It can be applied in an endoscopic approach by implementing PAM into a…

医学物理 · 物理学 2019-11-22 Zhendong Guo , Zhanhong Ye , Weihao Shao , Lili Jing , Sung-Liang Chen

As an important branch of photoacoustic microscopy, optical-resolution photoacoustic microscopy suffers from limited depth of field due to the strongly focused laser beam. In this work, a 3D information fusion algorithm based on 3D…

图像与视频处理 · 电气工程与系统科学 2022-11-29 Xianlin Song , Sihang Li , Zhuangzhuang Wang

Acoustic-resolution photoacoustic microscopy (AR-PAM) is a promising tool for microvascular imaging. In the focal region, resolution of AR-PAM is determined by the ultrasound transducer and ultimately limited by acoustic diffraction. In the…

图像与视频处理 · 电气工程与系统科学 2021-11-22 Fei Feng , Siqi Liang , Sung-Liang Chen

We developed a multifocus optical-resolution photoacoustic microscope (MPAM) with extended depth of field (DoF) by using a tunable acoustic gradient (TAG) lens and a pulse delay method. TAG lens is designed to control the divergence angle…

光学 · 物理学 2020-09-09 Xianlin Song

Photoacoustic microscopy (PAM) is a novel implementation of photoacoustic imaging (PAI) for visualizing the 3D bio-structure, which is realized by raster scanning of the tissue. However, as three involved critical imaging parameters,…

图像与视频处理 · 电气工程与系统科学 2023-08-10 Zhengyuan Zhang , Haoran Jin , Zesheng Zheng , Wenwen Zhang , Wenhao Lu , Feng Qin , Arunima Sharma , Manojit Pramanik , Yuanjin Zheng

We developed an optical-resolution photoacoustic microscope (OR-PAM) capable of changing focal plane with high-speed by using a tunable acoustic gradient (TAG) lens. In our system, a TAG lens is designed to continuously changing the focal…

光学 · 物理学 2020-09-09 Xianlin Song

Acoustic-Resolution Photoacoustic Microscopy (AR-PAM) is promising for subcutaneous vascular imaging, but its spatial resolution is constrained by the Point Spread Function (PSF). Traditional deconvolution methods like Richardson-Lucy and…

计算机视觉与模式识别 · 计算机科学 2024-10-29 Youshen Xiao , Sheng Liao , Xuanyang Tian , Fan Zhang , Xinlong Dong , Yunhui Jiang , Xiyu Chen , Ruixi Sun , Yuyao Zhang , Fei Gao

Optical resolution photoacoustic microscopy (OR-PAM) leverages optical focusing and acoustic detection for microscopic optical absorption imaging. Intrinsically it owns high optical lateral resolution and poor acoustic axial resolution.…

信号处理 · 电气工程与系统科学 2023-09-19 Yu-Hsiang Yu , Meng-Lin Li

Fourier ptychography microscopy (FPM), sharing its roots with synthetic aperture technique and phase retrieval method, is a recently developed computational microscopic super-resolution technique. By turning on the light-emitting diode…

光学 · 物理学 2021-05-07 G. Zhou , S. Zhang , C. Zheng , T. Li , Y. Hu , Q. Hao

One primary technical challenge in photoacoustic microscopy (PAM) is the necessary compromise between spatial resolution and imaging speed. In this study, we propose a novel application of deep learning principles to reconstruct…

图像与视频处理 · 电气工程与系统科学 2020-06-02 Anthony DiSpirito , Daiwei Li , Tri Vu , Maomao Chen , Dong Zhang , Jianwen Luo , Roarke Horstmeyer , Junjie Yao

Background: Photoacoustic Microscopy (PAM) integrates optical and acoustic imaging, offering enhanced penetration depth for detecting optical-absorbing components in tissues. Nonetheless, challenges arise in scanning large areas with high…

医学物理 · 物理学 2023-12-15 Irem Loc , Mehmet Burcin Unlu

Photoacoustic microscopy (PAM) is an emerging imaging method combining light and sound. However, limited by the laser's repetition rate, state-of-the-art high-speed PAM technology often sacrifices spatial sampling density (i.e.,…

图像与视频处理 · 电气工程与系统科学 2021-04-09 Tri Vu , Anthony DiSpirito , Daiwei Li , Zixuan Zhang , Xiaoyi Zhu , Maomao Chen , Laiming Jiang , Dong Zhang , Jianwen Luo , Yu Shrike Zhang , Qifa Zhou , Roarke Horstmeyer , Junjie Yao

Photoacoustic microscopy (PAM) has been a promising biomedical imaging technology in recent years. However, the point-by-point scanning mechanism results in low-speed imaging, which limits the application of PAM. Reducing sampling density…

图像与视频处理 · 电气工程与系统科学 2020-06-09 Jiasheng Zhou , Da He , Xiaoyu Shang , Zhendong Guo , Sung-liang Chen , Jiajia Luo

Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…

Aberration-corrected scanning transmission electron microscopes (STEM) provide sub-angstrom lateral resolution; however, the large convergence angle greatly reduces the depth of field. For microscopes with a small depth of field,…

材料科学 · 物理学 2011-12-15 Robert Hovden , Huolin L. Xin , David A. Muller

Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…

Current optoacoustic microscopy configurations often have narrow focal ranges that limit their use for fast volumetric imaging applications. Here, the focal range of optoacoustic microscopes is extended by matching the elongated optical…

光学 · 物理学 2021-04-15 Zakiullah Ali , Christian Zakian , Vasilis Ntziachristos
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