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Stroke poses a significant global health threat, with millions affected annually, leading to substantial morbidity and mortality. Current stroke risk assessment for the general population relies on markers such as demographics, blood tests,…

图像与视频处理 · 电气工程与系统科学 2024-07-24 Yu Xi Huang , Simon Mahler , Aidin Abedi , Julian Michael Tyszka , Yu Tung Lo , Patrick D. Lyden , Jonathan Russin , Charles Liu , Changhuei Yang

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

White blood cells (WBCs) play a crucial role in safeguarding the human body against pathogens and foreign substances. Leveraging the abundance of WBC imaging data and the power of deep learning algorithms, automated WBC analysis has the…

图像与视频处理 · 电气工程与系统科学 2023-10-23 Aditya Shankar Pal , Debojyoti Biswas , Joy Mahapatra , Debasis Banerjee , Prantar Chakrabarti , Utpal Garain

We present a dual color laser scanning endomicroscope capable of fluorescence lifetime endomicroscopy at one frame per second (FPS). The scanning system uses a coherent imaging fiber with 30,000 cores. High-speed lifetime imaging is…

Fiber-form optics extends the high-resolution tomographic imaging capabilities of Optical Coherence Tomography (OCT) to the inside of the human body, i.e., endoscopic OCT. However, it still faces challenges due to the trade-off between…

光学 · 物理学 2024-09-04 Chengfu Gu , Haoran Zhang , Qi Lan , Weiyi Zhang , Chang Liu , Jianlong Yang

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

定量方法 · 定量生物学 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis. Automatic detection, classification and counting of various…

计算机视觉与模式识别 · 计算机科学 2022-01-19 Xing Yao , Vinayak Pathak , Haoran Xi , Amey Chaware , Colin Cooke , Kanghyun Kim , Shiqi Xu , Yuting Li , Timothy Dunn , Pavan Chandra Konda , Kevin C. Zhou , Roarke Horstmeyer

Nonunion is one of the challenges faced by orthopedics clinics for the technical difficulties and high costs in photographing interosseous capillaries. Segmenting vessels and filling capillaries are critical in understanding the obstacles…

图像与视频处理 · 电气工程与系统科学 2022-07-15 Runpeng Hou , Ziyuan Ye , Chengyu Yang , Linhao Fu , Chao Liu , Quanying Liu

Noninvasive, non-contact and \textit{in vivo} monitoring of blood glucose is a long needed pathology tool for saving patients from recurring pain and hassle that can accompany conventional blood glucose testing methods. Optical coherence…

生物物理 · 物理学 2008-08-04 R Poddar , S R Sharma , P Sen , J T Andrews

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

光学 · 物理学 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of…

定量方法 · 定量生物学 2024-03-29 Yow-Ren Chang , Seong-Min Kim , Young Jong Lee

Due to the complex of mixed spectral point spread function within memory effect range, it is unreliable and slow to use speckle correlation technology for non-invasive imaging through scattering medium under broadband illumination. The…

光学 · 物理学 2023-05-03 Yunong Sun , Jianbin Liu , Hui Chen , Zhuoran Xi , Yu Zhou , Yuchen He , Huaibin Zheng , Zhuo Xu , Yuan Yuan

Exposure to laser light alters cell culture examination via optical microscopic imaging techniques, also based on label-free coherent digital holography. To mitigate this detrimental feature, researchers tend to use a broader spectrum and…

Breathing rate is a vital health metric that is an invaluable indicator of the overall health of a person. In recent years, the non-contact measurement of health signals such as breathing rate has been a huge area of development, with a…

图像与视频处理 · 电气工程与系统科学 2023-11-16 Robyn Maxwell , Timothy Hanley , Dara Golden , Adara Andonie , Joseph Lemley , Ashkan Parsi

In this chapter a general mathematical model of Optical Coherence Tomography (OCT) is presented on the basis of the electromagnetic theory. OCT produces high resolution images of the inner structure of biological tissues. Images are…

数值分析 · 数学 2016-04-19 Peter Elbau , Leonidas Mindrinos , Otmar Scherzer

High-numerical-aperture (NA) oblique plane microscopy enables noninvasive fluorescence imaging of subcellular dynamics without requiring radical sample modification. However, performance degrades at depth in multicellular specimens as…

Anterior uveitis, a common form of eye inflammation, can lead to permanent vision loss if not promptly diagnosed. Monitoring this condition involves quantifying inflammatory cells in the anterior chamber (AC) of the eye, which can be…

图像与视频处理 · 电气工程与系统科学 2024-06-26 Boyu Chen , Ameenat L. Solebo , Paul Taylor

Post-endoscopic gastrointestinal (GI) rebleeding frequently occurs within the first 72 hours after therapeutic hemostasis and remains a major cause of early morbidity and mortality. Existing non-invasive monitoring approaches primarily…

图像与视频处理 · 电气工程与系统科学 2026-01-27 Yunhao Bian , Dawei Wang , Mingyang Shen , Xinze Li , Jiayi Shi , Ziyao Zhou , Tiancheng Cao , Hen-Wei Huang

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…