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Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

A dual-excitation method for resonant-frequency tracking in scanning probe microscopy based on amplitude detection is developed. This method allows the cantilever to be operated at or near resonance for techniques where standard phase…

材料科学 · 物理学 2008-03-13 B J Rodriguez , C Callahan , S V Kalinin , R Proksch

We adjust the transient dynamics of a piezo-actuated bimorph Atomic Force Microscopy (AFM) probe using a state feedback controller. This approach enables us to adjust the quality factor and the resonance frequency of the probe…

原子物理 · 物理学 2012-04-16 Bilal Orun , Serkan Necipoglu , Cagatay Basdogan , Levent Guvenc

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

Myosin-II's rod-like tail drives filament assembly with a head arrangement that should generate equal and opposite contractile forces on actin--if one assumes that the filament is a symmetric bipole. Self-assembled myosin filaments are…

生物大分子 · 定量生物学 2008-11-20 Andre E. X. Brown , Alina Hategan , Daniel Safer , Yale E. Goldman , Dennis E. Discher

We demonstrate the application of Atomic Force Microscopy (AFM) based optical force microscopy to map the optical near-fields with nanometer resolution, limited only by the AFM probe geometry. We map the electric field distributions of…

Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent…

定量方法 · 定量生物学 2009-11-10 R. V. Krishnan , A. Masuda , V. E. Centoze , B. Herman

Analog computing at the edge is an emerging strategy to limit data storage and transmission requirements, as well as energy consumption, and its practical implementation is in its initial stages of development. Translating properties of…

信号处理 · 电气工程与系统科学 2025-12-09 Giuseppe Leo , Paolo Gibertini , Irem Ilter , Erika Covi , Ole Richter , Elisabetta Chicca

The plasma membrane of living cells is compartmentalized at multiple spatial scales ranging from the nano- to the meso-scale. This non-random organization is crucial for a large number of cellular functions. At the nanoscale, cell membranes…

In traditional quantum optics, where the interaction between atoms and light at optical frequencies is studied, the atoms can be approximated as point-like when compared to the wavelength of light. So far, this relation has also been true…

量子物理 · 物理学 2014-08-06 Anton Frisk Kockum , Per Delsing , Göran Johansson

Mechanical properties of biological samples have been imaged with a \textit{Force Feedback Microscope}. Force, force gradient and dissipation are measured simultaneously and quantitatively, merely knowing the AFM cantilever spring constant.…

生物物理 · 物理学 2014-05-01 Luca Costa , Mario S Rodrigues , Emily Newman , Chloe Zubieta , Joel Chevrier , Fabio Comin

The mechanical properties of collagen fibrils depend on the amount and the distribution of water molecules within the fibrils. Here, we use atomic force microscopy (AFM) to study the effect of hydration on the viscoelastic properties of…

软凝聚态物质 · 物理学 2019-10-03 Manuel R. Uhlig , Robert Magerle

We analyze an advanced two-spring model with an ultra-low effective tip mass to predict nontrivial and physically rich 'fine structure' in the atomic stick-slip motion in Friction Force Microscopy (FFM) experiments. We demonstrate that this…

软凝聚态物质 · 物理学 2009-11-13 D. Abel , S. Yu. Krylov , J. W. M. Frenken

Atomic Force Microscopy (AFM) allows to reconstruct the topography of surface with a resolution in the nanometer range. The exceptional resolution attainable with the AFM makes this instrument a key tool in nanoscience and technology. The…

Atomic force microscopy (AFM) is a powerful tool to investigate interaction forces at the micro and nanoscale. Cantilever stiffness, dimensions and geometry of the tip can be chosen according to the requirements of the specific application,…

应用物理 · 物理学 2020-07-31 M. Chighizola , L. Puricelli , L. Bellon , A. Podestà

Optically-active spin qubits have emerged as powerful quantum sensors capable of nanoscale magnetometry, yet conventional coherent sensing approaches are ultimately limited by the coherence time of the sensor, typically precluding detection…

量子物理 · 物理学 2025-10-10 Nicole Voce , Paul Stevenson

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

图像与视频处理 · 电气工程与系统科学 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

Quantifying the tip-sample interaction at the nanoscale in Amplitude Modulation mode AFM is challenging, especially when measuring in liquids. Here, we derive formulas for the tip-sample conservative and dissipative interactions and…

仪器与探测器 · 物理学 2015-11-24 Luca Costa , Mario S Rodrigues

We describe a first principles method to calculate scanning tunneling microscopy (STM) images, and compare the results to well-characterized experiments combining STM with atomic force microscopy (AFM). The theory is based on density…

介观与纳米尺度物理 · 物理学 2018-09-14 Alexander Gustafsson , Norio Okabayashi , Angelo Peronio , Franz J. Giessibl , Magnus Paulsson