相关论文: Spatio-angular fluorescence microscopy II. Paraxia…
The technique of remote refocusing is used in optical microscopy to provide rapid axial scanning without mechanically perturbing the sample and in techniques such as oblique plane microscopy that build on remote refocusing to image a tilted…
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…
We study the fundamental limits of detection for astrophysical observations in the far infrared. Understanding these fundamental limits is critical to the planning and analysis of experiments in this region. We specifically characterize the…
The thin and flexible nature of optical fibres often makes them the ideal technology to view biological processes in-vivo, but current microendoscopic approaches are limited in spatial resolution. Here, we demonstrate a new route to high…
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…
We present a plenoptic microscopy configuration for 3D snapshot imaging, which is dual telecentric and can directly record true projection images corresponding with different viewing angles. It also allows blocking high-angle stray rays…
In recent years, a great deal of emphasis has been placed on achieving the diffraction limit with large aperture telescopes. For a well matched focal-plane instrument, the diffraction limit provides the highest possible angular resolution…
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and…
Fiber-based confocal endomicroscopy has shown great promise for minimally-invasive deep-tissue imaging. Despite its advantages, confocal fiber-bundle endoscopy inherently suffers from undersampling due to the spacing between fiber cores,…
We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarization of the illumination or the detection…
We present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. From the measurement of a transmission matrix during a prior calibration step, a focused spot is produced and…
The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…
Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…
Imaging energy filters in photoelectron microscopes and momentum microscopes use spherical fields with deflection angles of 90{\deg}, 180{\deg}, and even 2 x 180{\deg}. These instruments are optimized for high energy resolution, but exhibit…
In the case of ground-based telescopes equipped with adaptive optics systems, the point spread function (PSF) is only poorly known or completely unknown. Moreover, an accurate modeling of the PSF is in general not available. Therefore in…
Modern extreme adaptive optics (AO) systems achieving diffraction-limited performance open up new possibilities for instrumentation. Especially important for the fields of spectroscopy and interferometry is that it enables the prospect to…
In this work, we study the conditions under which fluorescent beads can be used to emulate single fluorescent molecules in the calibration of optical microscopes. Although beads are widely used due to their brightness and easy manipulation,…
An optical field will undergo coherent diffusion when it is mapped into thermal-motioned atoms, e.g., in a slow or storage light process. As was demonstrated before, such diffusion effect is equivalent to a spatial low-pass filter…
MINFLUX microscopy allows for localization of fluorophores with nanometer precision using targeted scanning with an illumination profile with a minimum. However, current scanning patterns and the overall procedure are based on heuristics,…
[Abridged] We have developed an end-to-end simulation to specify the science requirements of a MOAO-fed integral field spectrograph on either an 8m or 42m telescope. Our simulations re-scales observations of local galaxies or results from…