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Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

光学 · 物理学 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Inhomogeneities in the refractive index of a biological sample can introduce phase aberrationsin microscopy systems, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve…

Light scattering and aberrations limit optical microscopy in biological tissue, which motivates the development of adaptive optics techniques. Here, we develop a method for adaptive optics with reflected light and deep neural networks…

光学 · 物理学 2020-07-28 Ivan Vishniakou , Johannes D. Seelig

Diffraction-limited imaging through complex scattering media is a long sought after goal with important applications in biomedical research. In recent years, high resolution wavefront-shaping has emerged as a powerful approach to generate a…

光学 · 物理学 2014-10-17 Ori Katz , Eran Small , Yefeng Guan , Yaron Silberberg

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

图像与视频处理 · 电气工程与系统科学 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

High-throughput 2D and 3D scanning electron microscopy, which relies on automation and dependable control algorithms, requires high image quality with minimal human intervention. Classical focus and astigmatism correction algorithms attempt…

仪器与探测器 · 物理学 2023-05-10 Philipp Johannes Schubert , Rangoli Saxena , Joergen Kornfeld

Light scattering inhibits high-resolution optical imaging, manipulation and therapy deep inside biological tissue by preventing focusing. To form deep foci, wavefront-shaping and time-reversal techniques that break the optical diffusion…

光学 · 物理学 2015-06-04 Cheng Ma , Fengbo Zhou , Yan Liu , Lihong V. Wang

Passive optical elements can play key roles in photonic applications such as plasmonic integrated circuits. Here we experimentally demonstrate passive gap-plasmon focusing and routing in two-dimensions. This is accomplished using a high…

Adaptive optics can focus light through opaque media by compensating the random phase delay acquired while crossing a scattering curtain. The technique is commonly exploited in many fields, including astrophysics, microscopy, biomedicine…

光学 · 物理学 2015-06-24 Diego Di Battista , Giannis Zacharakis , Marco Leonetti

The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the…

This article presents a method to perform diffraction tomography in a standard microscope that includes an LED array for illumination. After acquiring a sequence of intensity-only images of a thick sample, a ptychography-based…

光学 · 物理学 2015-10-30 Roarke Horstmeyer , Changhuei Yang

Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably…

光学 · 物理学 2025-03-24 D. W. S. Cox , T. Knop , I. M. Vellekoop

The major cornerstone of future ground-based astronomy is imaging and spectroscopy at the diffraction limit using adaptive optics. To exploit the potential of current AO systems, we have begun a survey around bright stars to study…

天体物理学 · 物理学 2007-05-23 R. I. Davies , M. Lehnert , A. J. Baker , S. Rabien

Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…

Perfect lensing using negative refractive index materials and radiationless electromagnetic interference both provide extreme subwavelength focusing by "amplifying" evanescent wave components that are usually lost. This paper provides a…

光学 · 物理学 2015-06-03 Reuven Gordon

Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…

In astronomy or biological imaging, refractive index inhomogeneities of e.g. atmosphere or tissues induce optical aberrations which degrade the desired information hidden behind the medium. A standard approach consists in measuring these…

光学 · 物理学 2023-06-01 Tengfei Wu , Marc Guillon , Gilles Tessier , Pascal Berto

We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle…

光学 · 物理学 2023-07-19 Hari P. Paudel , John Taranto , Jerome Mertz , Thomas Bifano

Scanning X-ray nanodiffraction microscopy is a powerful technique for spatially resolving nanoscale structural morphologies by diffraction contrast. One of the critical challenges in experimental nanodiffraction data analysis is posed by…

应用物理 · 物理学 2024-06-26 Aileen Luo , Tao Zhou , Martin V. Holt , Andrej Singer , Mathew J. Cherukara

We describe a diffraction microscopy technique based on refractive optics to study structural variations in crystals. The X-ray beam diffracted by a crystal was magnified by beryllium parabolic refractive lenses on a 2D X-ray camera. The…

光学 · 物理学 2014-12-16 Thomas Roth , Carsten Detlefs , Irina Snigireva , Anatoly Snigirev
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