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The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

In numerous industrial processes involving fluids, viscosity is a determinant factor for reaction rates, flows, drying, mixing, etc. Its importance is even more determinant for phenomena observed are at the micro- and nano- scales as in…

We present a theoretical model to sufficiently investigate the optical rotational Doppler effect based on modal expansion method. We find that the frequency shift content is only determined by the surface of spinning object and the reduced…

光学 · 物理学 2016-08-02 Hailong Zhou , Dongzhi Fu , Jianji Dong , Pei Zhang , Xinliang Zhang

Fluctuation in fluorescence emission of immobilized single molecule is typically ascribed to the chromophore's intrinsic structural conformations and the influence of local environmental factors. Despite extensive research over several…

化学物理 · 物理学 2024-08-07 Aranyak Sarkar , Vinu Namboodiri , Manoj Kumbhakar

Recording the fluorescence of a magneto-optical trap (MOT) is a standard tool for measuring atom numbers in experiments with ultracold atoms. When trapping few atoms in a small MOT, the emitted fluorescence increases with the atom number in…

量子气体 · 物理学 2021-03-17 Martin Schlederer , Alexandra Mozdzen , Thomas Lompe , Henning Moritz

The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…

介观与纳米尺度物理 · 物理学 2010-07-22 Michele Celebrano , Philipp Kukura , Alois Renn , Vahid Sandoghdar

Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…

Rotational dynamics often challenge physical intuition while enabling unique realizations, from the rotor of a gyroscope that maintains its orientation regardless of the outer gimbals, to a tennis racket that rotates around its handle when…

We computationally demonstrate a new method for coherently controlling the rotation-axis direction in asymmetric top molecules with an optical centrifuge. Appropriately chosen electric-field strengths and the centrifuge's acceleration rate…

化学物理 · 物理学 2021-06-16 Emil J. Zak , Andrey Yachmenev , Jochen Küpper

Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…

生物物理 · 物理学 2011-12-23 Charles Edouard Leroux , Irène Wang , Jacques Derouard , Antoine Delon

Molecule-surface interaction is key to many physical and chemical processes at interfaces. Here, we show that the dynamics of single molecules on a surface under ultrahigh vacuum can be resolved using fluorescence imaging. By adapting…

化学物理 · 物理学 2021-10-19 Tianyu Fang , Florian Elsen , Nick Vogeley , Daqing Wang

We investigate the properties of a single-view fluorescence microscope in a 4$f$ geometry when imaging fluorescent dipoles without using the monopole or scalar approximations. We show that this imaging system has a spatio-angular band…

光学 · 物理学 2019-07-16 Talon Chandler , Hari Shroff , Rudolf Oldenbourg , Patrick La Rivière

We propose and demonstrate a novel technique that combines Raman scattering and optical cycling in molecules with diagonal Franck-Condon factors. This resonance Raman optical cycling manipulates molecules to behave like efficient…

原子物理 · 物理学 2021-08-26 J. C. Shaw , J. C. Schnaubelt , D. J. McCarron

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

生物大分子 · 定量生物学 2015-04-15 Mark Leake

When circularly polarized light is scattered from a rotating target, a rotational Doppler shift (RDS) emerges from an exchange of angular momentum between the spinning object and the electromagnetic field. Here, we used coherently spinning…

A beam of rotating dipolar particles (molecules or clusters) will broaden when passed through an electric or magnetic field gradient region. This broadening, which is a common experimental observable, can be expressed in terms of the…

原子与分子团簇 · 物理学 2012-01-05 Jaap Bulthuis , Vitaly V. Kresin

Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…

生物物理 · 物理学 2017-05-08 Martin Lindén , Vladimir Ćurić , Elias Amselem , Johan Elf

A variety of recent imaging techniques are able to beat the diffraction limit in fluorescence microcopy by activating and localizing subsets of the fluorescent molecules in the specimen, and repeating this process until all of the molecules…

光学 · 物理学 2010-08-27 Alexander R. Small

We demonstrate experimentally a method of varying the degree of directionality in laser-induced molecular rotation. To control the ratio between the number of clockwise and counter-clockwise rotating molecules (with respect to a fixed…

化学物理 · 物理学 2021-05-05 Alexander A. Milner , Valery Milner

We theoretically investigate the optical rotational Doppler Effect using modal expansion method. We find that the frequency shift content is only determined by the surface of spinning object and the reduced Doppler shift is linear to the…

光学 · 物理学 2015-07-16 Hailong Zhou , Jianji Dong , Pei Zhang , Xinliang Zhang