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The self-organization of proteins into enriched compartments and the formation of complex patterns are crucial processes for life on the cellular level. Liquid-liquid phase separation is one mechanism for forming such enriched compartments.…

软凝聚态物质 · 物理学 2025-04-07 Antonia Winter , Yuhao Liu , Alexander Ziepke , George Dadunashvili , Erwin Frey

This article summarizes a variety of physical mechanisms proposed in the literature, which can generate micro- and nanodomains in multicomponent lipid bilayers and biomembranes. It mainly focusses on lipid-driven mechanisms that do not…

生物物理 · 物理学 2016-11-16 Friederike Schmid

Heterogeneities in the cell membrane due to coexisting lipid phases have been conjectured to play a major functional role in cell signaling and membrane trafficking. Thereby the material properties of multiphase systems, such as the line…

软凝聚态物质 · 物理学 2013-04-17 Stefan Semrau , Timon Idema , Laurent Holtzer , Thomas Schmidt , Cornelis Storm

Biological membranes constitute boundaries of cells and cell organelles. Physico-chemical mechanisms at the atomic scale are dictated by protein-lipid interaction strength, lipid composition, lipid distribution in the vicinity of the…

生物物理 · 物理学 2015-10-19 N. Ramakrishnan , P. B. Sunil Kumar , Ravi Radhakrishnan

We study the phase separation of binary lipid mixtures that form bicontinuous cubic phases. The competition between non-uniform Gaussian membrane curvature and line tension leads to a very rich phase diagram, where we observe symmetry…

软凝聚态物质 · 物理学 2016-08-24 Fabien Paillusson , Matthew R. Pennington , Halim Kusumaatmaja

We study two mechanisms for the formation of protein patterns near membranes of living cells by mathematical modelling. Self-assembly of protein domains by electrostatic lipid-protein interactions is contrasted with self-organization due to…

细胞行为 · 定量生物学 2007-05-23 Karin John , Markus Baer

Giant unilamellar vesicles (GUVs) composed of as few as three lipid species can phase separate into small-scale lipid domains with stripes and dots patterns. These patterns have been experimentally characterized in terms of how their size…

软凝聚态物质 · 物理学 2025-05-21 Qiwei Yu , Andrej Košmrlj

In experiments on model membranes, a formation of large domains of different lipid composition is readily observed. However, no such phase separation is observed in the membranes of intact cells. Instead, a structure of small transient…

软凝聚态物质 · 物理学 2017-08-23 Timo Fischer , H. Jelger Risselada , Richard L. C. Vink

Phase separation within polymer networks plays a central role in shaping the structure and mechanics of both synthetic materials and living cells, including the formation of biomolecular condensates within cytoskeletal networks. Previous…

软凝聚态物质 · 物理学 2025-12-01 Takahiro Yokoyama , Yicheng Qiang , David Zwicker , Arash Nikoubashman

We discuss a realistic scenario, accounting for the existence of sub-micrometric protein domains in cell membranes. At the biological level, such membrane domains have been shown to be specialized, in order to perform a determined…

软凝聚态物质 · 物理学 2015-05-14 Nicolas Destainville

A novel two-leaflet description of lipid membranes is proposed. Within its framework, phase separation phenomena in multicomponent biological membranes are analyzed. As we show, interactions between the leaflets tend to suppress macroscopic…

生物物理 · 物理学 2016-02-03 Ramon Reigada , Alexander S. Mikhailov

Liposomes that achieve a heterogeneous and spatially organized surface through phase separation have been recognized to be a promising platform for delivery purposes. However, their design and optimization through experimentation can be…

软凝聚态物质 · 物理学 2021-11-05 Y. Wang , Y. Palzhanov , A. Quaini , M. Olshanskii , S. Majd

Multi-component membranes such as ternary mixtures of lipids and cholesterol can exhibit coexistence regions between two liquid phases. When such membranes adhere to a corrugated substrate, the phase separation process strongly depends on…

软凝聚态物质 · 物理学 2009-11-13 Bartosz Rozycki , Thomas R. Weikl , Reinhard Lipowsky

The formation of polarized signaling domains on cell membranes is a fundamental example of biological pattern formation. While such patterns resemble structures from equilibrium phase separation, they are intrinsically non-equilibrium,…

生物物理 · 物理学 2025-12-10 Damiano Andreghetti , Alfredo Braunstein , Luca Dall'Asta , Andrea Gamba

Biomembranes, which are mainly composed of neutral and charged lipids, exhibit a large variety of functional structures and dynamics. Here, we report a coarse-grained molecular dynamics (MD) simulation of the phase separation and…

软凝聚态物质 · 物理学 2016-11-07 Hiroaki Ito , Yuji Higuchi , Naofumi Shimokawa

Lipid vesicles composed of a mixture of two types of lipids are studied by intensive Monte-Carlo numerical simulations. The coupling between the local composition and the membrane shape is induced by two different spontaneous curvatures of…

软凝聚态物质 · 物理学 2020-07-15 Julie Cornet , Nicolas Destainville , Manoel Manghi

Cellular membranes are a heterogeneous mix of lipids, proteins and small molecules. Special groupings of saturated lipids and cholesterol form a liquid-ordered phase, known as `lipid rafts,' serving as platforms for signaling, trafficking…

定量方法 · 定量生物学 2015-05-13 Tristan S. Ursell , William S. Klug , Rob Phillips

Ever since the raft model for biomembranes has been proposed, the traditional view of biomembranes based on the fluid-mosaic model has been altered. In the raft model, dynamical heterogeneities in multi-component lipid bilayers play an…

软凝聚态物质 · 物理学 2014-10-16 Shigeyuki Komura , David Andelman

Unravelling the physical mechanisms behind the organisation of lipid domains is a central goal in cell biology and membrane biophysics. Previous studies on cells and model lipid bilayers featuring phase-separated domains found an intricate…

软凝聚态物质 · 物理学 2018-04-24 Melissa Rinaldin , Piermarco Fonda , Luca Giomi , Daniela J. Kraft

Plasma membranes appear as deformable systems wherein molecules are free to move and diffuse giving rise to condensed microdomains (composed of ordered lipids, transmembrane proteins and cholesterol) surrounded by disordered lipid…

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