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The wavelength calibration of spectrographs is an essential but challenging task in many disciplines. Calibration is traditionally accomplished by imaging the spectrum of a light source containing features that are known to appear at…

天体物理仪器与方法 · 物理学 2018-04-04 Neil Thomas

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

光学 · 物理学 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian

We demonstrate the possibility of a self-consistent characterization of the photon-number statistics of a light field by using photoemissive detectors with internal gain simply endowed with linear input/output responses. The method can be…

量子物理 · 物理学 2015-05-13 Maria Bondani , Alessia Allevi , Andrea Agliati , Alessandra Andreoni

The propagation of errors through the uniform disk visibility function is examined. Implications of those errors upon measures of absolute visibility through optical and near-infrared interferometers are considered within the context of…

天体物理学 · 物理学 2009-11-11 Gerard T. van Belle , Gerald van Belle

We describe the optimum telescope focal ratio for a two-element, three surface, telecentric image-transfer microlens-to-fiber coupled integral field unit within the constraints imposed by micro-optics fabrication and optical aberrations. We…

天体物理仪器与方法 · 物理学 2022-05-09 Sabyasachi Chattopadhyay , Matthew A. Bershady , Marsha J. Wolf , Michael P. Smith

The complexity and accuracy of current and future precision cosmology observational campaigns has made it essential to develop an efficient technique for directly combining simulation and observational datasets to determine cosmological and…

天体物理学 · 物理学 2009-11-11 Katrin Heitmann , David Higdon , Charles Nakhleh , Salman Habib

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…

生物物理 · 物理学 2014-03-13 Charles-Edouard Leroux , Alexei Grichine , Irène Wang , Antoine Delon

Robust and accurate camera calibration is essential for 3D reconstruction in light microscopy under circular motion. Conventional methods require either accurate key point matching or precise segmentation of the axial-view images. Both…

计算机视觉与模式识别 · 计算机科学 2019-10-31 Yuanhao Guo , Fons J. Verbeek , Ge Yang

This paper describes the setup of two different solutions for laboratory X-ray microscopy working with geometric magnification. One setup uses thin-film transmission targets with an optimized tungsten-layer thickness and the electron gun…

仪器与探测器 · 物理学 2015-06-11 Thomas Ebensperger , Philipp Stahlhut , Frank Nachtrab , Simon Zabler , Randolf Hanke

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

光学 · 物理学 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

Aberration correctors are essential elements for achieving atomic resolution in state-of-the-art electron microscopes. Conventional correctors are based on a series of multipolar electron lenses, but more versatile alternatives are…

光学 · 物理学 2025-01-29 Zdeněk Nekula , Thomas Juffmann , Andrea Konečná

In this paper are reviewed various designs of advanced, multi-aperture optical systems dedicated to high angular resolution imaging or to the detection of exo-planets by nulling interferometry. A simple Fourier optics formalism applicable…

光学 · 物理学 2016-01-20 Francois Henault

In a line caustic crossing microlensing event, the caustic line moving across the surface of the source star provides a direct method to measure the integrated luminosity profile of the star. Combined with the enormous brightening at the…

天体物理学 · 物理学 2007-05-23 Sun Hong Rhie , David P. Bennett

Three-dimensional direct laser writing via two photon polymerization is used to fabricate anti-reflective structured surfaces composed of sub-wavelength conicoid features optimized to operate over a wide bandwidth in the near-infrared range…

光学 · 物理学 2018-01-08 Y. Li , D. B. Fullager , E. Angelbello , D. Childers , G. Boreman , T. Hofmann

We present a novel learning-based method to build a differentiable computational model of a real fluorescence microscope. Our model can be used to calibrate a real optical setup directly from data samples and to engineer point spread…

图像与视频处理 · 电气工程与系统科学 2023-06-12 Josue Page , Paolo Favaro

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

光学 · 物理学 2022-01-26 Yi Xue , David Ren , Laura Waller

We quantify the performance of mass mapping techniques on mock imaging and gravitational lensing data of galaxy clusters. The optimum method depends upon the scientific goal. We assess measurements of clusters' radial density profiles,…

宇宙学与河外天体物理 · 物理学 2020-07-29 Sut-Ieng Tam , Richard Massey , Mathilde Jauzac , Andrew Robertson

An efficient method to tune the spatial coherence of a degenerate laser over a broad range with minimum variation in the total output power is presented. It is based on varying the diameter of a spatial filter inside the laser cavity. The…

光学 · 物理学 2015-06-16 Micha Nixon , Brandon Redding , Asher Friesem , Hui Cao , Nir Davidson

Astrometric observations of microlensing events were originally proposed to determine the lens proper motion with which the physical parameters of lenses can be better constrained. In this proceeding, we demonstrate that besides this…

天体物理学 · 物理学 2007-05-23 Cheongho Han