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Length-regulation of microtubules (MTs) is essential for many cellular processes. Molecular motors like kinesin 8, which move along MTs and also act as depolymerases, are known as key players in MT dynamics. However, the regulatory…

亚细胞过程 · 定量生物学 2012-10-18 Anna Melbinger , Louis Reese , Erwin Frey

The cytoskeleton is regulated by a plethora of enzymes that influence the stability and dynamics of cytoskeletal filaments. Molecular motors of the kinesin-8 protein family depolymerise microtubules in a length-dependent manner, and…

亚细胞过程 · 定量生物学 2014-10-29 Louis Reese , Anna Melbinger , Erwin Frey

Proteins from the kinesin-8 family promote microtubule (MT) depolymerization, a process thought to be important for the control of microtubule length in living cells. In addition to this MT shortening activity, kinesin 8s are motors that…

生物物理 · 物理学 2015-05-13 L. E. Hough , Anne Schwabe , Matthew A. Glaser , J. Richard McIntosh , M. D. Betterton

Regulating physical size is an essential problem that biological organisms must solve from the subcellular to the organismal scales, but it is not well understood what physical principles and mechanisms organisms use to sense and regulate…

生物物理 · 物理学 2014-07-21 Hui-Shun Kuan , M. D. Betterton

Assembly and disassembly dynamics of microtubules (MTs) is tightly controlled by MT associated proteins. Here, we investigate how plus-end-directed depolymerases of the kinesin-8 family regulate MT depolymerization dynamics. Employing an…

生物物理 · 物理学 2011-11-15 Louis Reese , Anna Melbinger , Erwin Frey

In this work we study a microtubule (MT) model, whose length is regulated by the action of processive kinesin motors. We treat the case of infinite processivity, i.e. particle exchange in the bulk is neglected. The exact results can be…

统计力学 · 物理学 2015-06-25 Chikashi Arita , Alexander Lück , Ludger Santen

In many intracellular processes, the length distribution of microtubules is controlled by depolymerizing motor proteins. Experiments have shown that, following non-specific binding to the surface of a microtubule, depolymerizers are…

生物物理 · 物理学 2009-11-13 Bindu S. Govindan , Manoj Gopalakrishnan , Debashish Chowdhury

The microtubule cytoskeleton is responsible for sustained, long-range intracellular transport of mRNAs, proteins, and organelles in neurons. Neuronal microtubules must be stable enough to ensure reliable transport, but they also undergo…

动力系统 · 数学 2024-03-05 Anna C Nelson , Melissa M Rolls , Maria-Veronica Ciocanel , Scott A McKinley

We formulate and analyze a theoretical model for the regulation of microtubule (MT) polymerization dynamics by the signaling proteins Rac1 and stathmin. In cells, the MT growth rate is inhibited by cytosolic stathmin, which, in turn, is…

亚细胞过程 · 定量生物学 2014-12-18 Maria Zeitz , Jan Kierfeld

In living cells, motor proteins, such as kinesin and dynein can move processively along microtubule (MT), and also detach from or attach to MT stochastically. Experiments have found that, the traffic of motor might be jammed, and various…

生物物理 · 物理学 2015-03-06 Yunxin Zhang

Assembly and stability of mitotic spindle is governed by the interplay of various intra-cellular forces, e.g. the forces generated by motor proteins by sliding overlapping anti-parallel microtubules (MTs) polymerized from the opposite…

生物物理 · 物理学 2016-08-24 Paolo Malgaretti , Sudipto Muhuri

Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin 8s on microtubule…

亚细胞过程 · 定量生物学 2016-04-28 Zachary Gergely , Ammon Crapo , Loren E. Hough , J. Richard McIntosh , Meredith D. Betterton

Certain regulatory proteins influence the polymerization dynamics of microtubules by inducing catastrophe with a rate that depends on the microtubule length. Using a discrete formulation, here we show that, for a catastrophe rate…

生物物理 · 物理学 2012-08-27 Vandana Yadav , Sutapa Mukherji

Microtubules are highly regulated dynamic elements of the cytoskeleton of eukaryotic cells. One of the regulation mechanisms observed in living cells is the severing by the proteins katanin and spastin. We introduce a model for the dynamics…

亚细胞过程 · 定量生物学 2010-03-12 Simon H. Tindemans , Bela M. Mulder

Biomolecular motor proteins that generate forces by consuming chemical energy obtained from ATP hydrolysis are pivotal for organizing broad cytoskeletal structures in living cells. The control of such cytoskeletal structures benefits…

软凝聚态物质 · 物理学 2021-12-30 Shunya Araki , Kazusa Beppu , Arif Md. Rashedul Kabir , Akira Kakugo , Yusuke T. Maeda

The microtubule (MT) motor Kip3p is very processive kinesin that promotes catastrophes and pausing in particular on cortical contact. These properties explain the role of Kip3p in positioning the mitotic spindle in budding yeast and…

定量方法 · 定量生物学 2018-02-05 Chikashi Arita , Jonas Bosche , Alexander Lück , Ludger Santen

Microtubules are stiff filamentary proteins that constitute an important component of the cytoskeleton of cells. These are known to exhibit a dynamic instability. A steadily growing microtubule can suddenly start depolymerizing very…

统计力学 · 物理学 2009-11-10 Pankaj Kumar Mishra , Ambarish Kunwar , Sutapa Mukherji , Debashish Chowdhury

The cytoskeleton is essential for the maintenance of cell morphology in eukaryotes. In fission yeast for example, polarized growth sites are organized by actin whereas microtubules (MT) acting upstream control where growth occurs (La…

细胞行为 · 定量生物学 2009-03-30 Dietrich Foethke , Tatyana Makushok , Damian Brunner , Francois Nedelec

Conventional kinesin is a homodimeric motor protein that unidirectionally transports organelles along filamentous microtubule (MT) by hydrolyzing ATP molecules. This study shows that the load modulations of ATP turnover and head diffusion…

生物物理 · 物理学 2009-07-13 Dagong Fan , Wenwei Zheng , Ruizheng Hou , Fuli Li , Zhisong Wang

The cellular cytoskeleton is an active material, driven out of equilibrium by molecular motor proteins. It is not understood how the collective behaviors of cytoskeletal networks emerge from the properties of the network's constituent motor…

亚细胞过程 · 定量生物学 2017-12-19 Peter J. Foster , Wen Yan , Sebastian Fürthauer , Michael J. Shelley , Daniel J. Needleman
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