相关论文: Two-photon photoactivated voltage imaging in tissu…
We present a palette of brightly fluorescent genetically encoded voltage indicators (GEVIs) with excitation and emission peaks spanning the visible spectrum, sensitivities from 6 - 10% Delta F/F per 100 mV, and half-maximal response times…
Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…
High-speed optical imaging of dynamic neuronal activity is essential yet challenging in neuroscience. While calcium imaging has been firmly established as a workhorse technique for monitoring neuronal activity, its limited temporal…
Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz),…
Two-photon excitation in the near-infrared (NIR) of colloidal nanocrystalline silicon quantum dots (nc-SiQDs) with photoluminescence also in the NIR has the potential to open up new opportunities in the field of deep biological imaging.…
Coherent control is extended to macroscopic processes under continuous pulsed laser irradiation. Here, this approach is used to analyze the experimentally measured two-photon phase control of currents emanating from a living brain cells…
3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…
Wavefront-shaping is a promising approach for imaging fluorescent targets deep inside scattering tissue despite strong aberrations. It enables focusing an incoming illumination into a single spot inside tissue, as well as correcting the…
Photosensitive proteins embedded in the cell membrane (about 5 nm thickness) act as photoactivated proton pumps, ion gates, enzymes, or more generally, as initiators of stimuli for the cell activity. They are composed of a protein backbone…
Two-photon laser scanning microscopy is widely used in a quickly growing field of neuroscience. It is a fluorescence imaging technique that allows imaging of living tissue up to a very high depth to study inherent brain structure and…
We report a theoretical/computational approach for modeling the current-voltage characteristics of sensing proteins. The modeling is applied to a couple of transmembrane proteins, bacteriorhodopsin and proteorhodopsin, sensitive to visible…
We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During…
We developed a table-top vacuum ultraviolet (VUV) laser with $113.778$nm wavelength (10.897eV) and demonstrated its viability as a photon source for high resolution angle-resolved photoemission spectroscopy (ARPES). This sub-nanosecond…
Over the past decade, techniques enabling bidirectional modulation of neuronal activity with single cell precision have rapidly advanced in the form of two-photon optogenetic stimulation. Unlike conventional electrophysiological approaches…
Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…
Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution…
A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well-defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and…
Recently, we have proposed a redox molecular hypothesis about the natural biophysical substrate of visual perception and imagery (B\'okkon, 2009. BioSystems; B\'okkon and D'Angiulli, 2009. Bioscience Hypotheses). Namely, the retina…
An exciting recent development for deep-tissue imaging with cellular resolution is three-photon fluorescence microscopy (3PM) with excitation at long wavelengths (1300 and 1700 nm). In the last few years, long-wavelength 3PM has driven…
We combine generative adversarial network (GAN) with light microscopy to achieve deep learning super-resolution under a large field of view (FOV). By appropriately adopting prior microscopy data in an adversarial training, the neural…