相关论文: Sub-100 nm 3-D fluorescence lifetime imaging using…
Their intrinsic properties render single quantum systems as ideal tools for quantum enhanced sensing and microscopy. As an additional benefit, their size is typically on an atomic scale which enables sensing with very high spatial…
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…
Capturing depth and reflectivity images at low light levels from active illumination of a scene has wide-ranging applications. Conventionally, even with single-photon detectors, hundreds of photon detections are needed at each pixel to…
We reconstructed the 3D Fourier intensity distribution of mono-disperse prolate nano-particles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast X-ray pulse intercepted…
The spontaneous fluorescence rates of single-molecule emitters are typically on the order of nanoseconds. However coupling them with plasmonic nanostructures can substantially increase their fluorescence yields. The confinement between the…
Low-light-level imaging techniques have application in many diverse fields, ranging from biological sciences to security. We demonstrate a single-photon imaging system based on a time-gated inten- sified CCD (ICCD) camera in which the image…
We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…
Electron microscopy based on high-energy electrons allows nanoscopic analytical imaging taking advantage of secondarily generated particles. Especially for cathodoluminescence, the correlation between primary incident electrons and emitted…
The number of photons available by coherent X-ray scattering from a single biomolecule is considerably less because of the extremely small elastic-scattering cross-section and low damage threshold. Even with a high X-ray flux of 3 x 10to 12…
Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…
Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…
Multi-photon microscopy has played a significant role in biological imaging since it allows to observe living tissues with improved penetration depth and excellent sectioning effect. Multi-photon microscopy relies on multi-photon…
Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…
Active 3D imaging systems have broad applications across disciplines, including biological imaging, remote sensing and robotics. Applications in these domains require fast acquisition times, high timing resolution, and high detection…
This paper reported a bespoke adder-based deep learning network for time-domain fluorescence lifetime imaging (FLIM). By leveraging the l1-norm extraction method, we propose a 1-D Fluorescence Lifetime AdderNet (FLAN) without…
Long-range active imaging has widespread applications in remote sensing and target recognition. Single-photon light detection and ranging (lidar) has been shown to have high sensitivity and temporal resolution. On the application front,…
A 790-nm-driven high-harmonic generation source with a repetition rate of 6 kHz is combined with a toroidal-grating monochromator and a high-detection-efficiency photoelectron time-of-flight momentum microscope to enable time- and…
The development of single-photon counting detectors and arrays has made tremendous steps in recent years, not the least because of various new applications in, e.g., LIDAR devices. In this work, a 3D imaging device based on real thermal…