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Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

图像与视频处理 · 电气工程与系统科学 2022-10-20 Abhishek Mall , Kartik Ayyer

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

光学 · 物理学 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

Live-cell imaging of multiple subcellular structures is essential for understanding subcellular dynamics. However, the conventional multi-color sequential fluorescence microscopy suffers from significant imaging delays and limited number of…

亚细胞过程 · 定量生物学 2025-01-13 Mingyang Chen , Luhong Jin , Xuwei Xuan , Defu Yang , Yun Cheng , Ju Zhang

As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating dye YOYO-1 against minor groove binding dye SYTO-13, using a…

生物大分子 · 定量生物学 2015-04-15 Helen Miller , Zhaokun Zhou , Adam J. M. Wollman , Mark C. Leake

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…

光学 · 物理学 2025-01-17 David Alonso , Javier Garcia , Vicente Mico

Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…

We demonstrate an electro-optic wide-field method to enable fluorescence lifetime microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven Pockels cells are used to efficiently gate images at 39 MHz,…

光学 · 物理学 2021-09-23 Adam J. Bowman , Mark A. Kasevich

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

光学 · 物理学 2021-08-26 Zengbo Wang

The emergence of far-field super-resolution microscopy has rejuvenated the possibility for nanoscale imaging. Approaches to far-field super-resolution that utilize point scanning often depends on spatially reducing the size of the focused…

光学 · 物理学 2022-06-17 Ning Xu , Guoxuan Liu , Qiaofeng Tan

In example-based super-resolution, the function relating low-resolution images to their high-resolution counterparts is learned from a given dataset. This data-driven approach to solving the inverse problem of increasing image resolution…

图像与视频处理 · 电气工程与系统科学 2018-12-05 Alexander Robey , Vidya Ganapati

Magnetic resonance imaging (MRI) plays a vital role in clinical diagnostics, yet it remains hindered by long acquisition times and motion artifacts. Multi-contrast MRI reconstruction has emerged as a promising direction by leveraging…

计算机视觉与模式识别 · 计算机科学 2026-01-15 Xinming Fang , Chaoyan Huang , Juncheng Li , Jun Wang , Jun Shi , Guixu Zhang

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

光学 · 物理学 2022-01-26 Yi Xue , David Ren , Laura Waller

In traditional optical imaging systems, the spatial resolution is limited by the physics of diffraction, which acts as a low-pass filter. The information on sub-wavelength features is carried by evanescent waves, never reaching the camera,…

光学 · 物理学 2018-12-13 Oren Solomon , Yonina C. Eldar , Maor Mutzafi , Mordechai Segev

Nanophotonic devices seek to generate, guide, and/or detect light using structures whose nanoscale dimensions are closely tied to their functionality. Semiconducting nanowires, grown with tailored optoelectronic properties, have been…

介观与纳米尺度物理 · 物理学 2015-05-19 Patrick Irvin , Yanjun Ma , Daniela F. Bogorin , Cheng Cen , Chung Wung Bark , Chad M. Folkman , Chang-Beom Eom , Jeremy Levy

The paper presents an alternative way to classical stereocorrelation. First, 2D image processing of random patterns is described. Sub-pixel displacements are determined using phase analysis. Then distortion evaluation is presented. The…

经典物理 · 物理学 2013-11-18 Jérôme Molimard , Gaetan Boyer , Hassan Zahouani

The structured illumination microscopy using unknown speckle patterns has shown the capacity to surpass the Abbe's diffraction barrier, giving the possibility to design cheap and versatile SIM devices. However, the state-of-the-art joint…

图像与视频处理 · 电气工程与系统科学 2020-08-27 Penghuan Liu

Biosensing with ferromagnet-based magnetoresistive devices has been dominated by electrical detection of particle-induced changes to the devices' static magnetic configuration. There are however potential advantages to be gained from using…

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

光学 · 物理学 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

The low-density imaging performance of a zone plate based nano-resolution hard X-ray computed tomography (CT) system can be significantly improved by incorporating a grating-based Lau interferometer. Due to the diffraction, however, the…

图像与视频处理 · 电气工程与系统科学 2023-10-16 Xuebao Cai , Yuhang Tan , Ting Su , Dong Liang , Hairong Zheng , Jinyou Xu , Peiping Zhu , Yongshuai Ge