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Recent progress has boosted the resolving power of optical microscopies to spatial dimensions well below the diffraction limit. Yet, axial super-resolution and axial single-molecule localisation typically require more complicated…

Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…

光学 · 物理学 2017-08-02 Hsiou-Yuan Liu , Jingshan Zhong , Laura Waller

Single-molecule localization microscopy (SMLM) allows reconstructing biology-relevant structures beyond the diffraction limit by detecting and localizing individual fluorophores -- fluorescent molecules stained onto the observed specimen --…

计算机视觉与模式识别 · 计算机科学 2026-03-03 Romain Seailles , Jean-Baptiste Masson , Jean Ponce , Julien Mairal

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

光学 · 物理学 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

Masked video modeling, such as VideoMAE, is an effective paradigm for video self-supervised learning (SSL). However, they are primarily based on reconstructing pixel-level details on natural videos which have substantial temporal…

计算机视觉与模式识别 · 计算机科学 2025-04-02 Fida Mohammad Thoker , Letian Jiang , Chen Zhao , Bernard Ghanem

We present a novel approach to implement compressive sensing in laser scanning microscopes (LSM), specifically in image scanning microscopy (ISM), using a single-photon avalanche diode (SPAD) array detector. Our method addresses two…

图像与视频处理 · 电气工程与系统科学 2023-07-20 Ajay Gunalan , Marco Castello , Simonluca Piazza , Shunlei Li , Alberto Diaspro , Leonardo S. Mattos , Paolo Bianchini

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

图像与视频处理 · 电气工程与系统科学 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

High-resolution fMRI provides a window into the brain's mesoscale organization. Yet, higher spatial resolution increases scan times, to compensate for the low signal and contrast-to-noise ratio. This work introduces a deep learning-based 3D…

图像与视频处理 · 电气工程与系统科学 2024-03-20 Hongwei Bran Li , Matthew S. Rosen , Shahin Nasr , Juan Eugenio Iglesias

Given the hyper-spectral imaging unique potentials in grasping the polymer characteristics of different materials, it is commonly used in sorting procedures. In a practical plastic sorting scenario, multiple plastic flakes may overlap which…

计算机视觉与模式识别 · 计算机科学 2022-03-24 Guillem Martinez , Maya Aghaei , Martin Dijkstra , Bhalaji Nagarajan , Femke Jaarsma , Jaap van de Loosdrecht , Petia Radeva , Klaas Dijkstra

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

光学 · 物理学 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

On-chip optical architectures that enable angle-resolved spectroscopy are essential for advancing photonic platforms towards low-volume, scalable, and cryo-compatible devices. Here, we introduce spatially resolved momentum-space imaging…

As the rapid development of computer vision and the emergence of powerful network backbones and architectures, the application of deep learning in medical imaging has become increasingly significant. Unlike natural images, medical images…

图像与视频处理 · 电气工程与系统科学 2026-04-10 Guoqing Zhang , Jingyun Yang , Yang Li

Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multi-color capability with high-quality illumination is currently lacking.…

光学 · 物理学 2023-03-14 Ning Xu , Sarah E. Bohndiek , Zexing Li , Cilong Zhang , Qiaofeng Tan

In light-sheet microscopy, overall image content and resolution are improved by acquiring and fusing multiple views of the sample from different directions. State-of-the-art multi-view (MV) deconvolution employs the point spread functions…

定量方法 · 定量生物学 2015-03-30 Benjamin Schmid , Jan Huisken

The ability to extend the field of view of laparoscopy images can help the surgeons to obtain a better understanding of the anatomical context. However, due to tissue deformation, complex camera motion and significant three-dimensional (3D)…

计算机视觉与模式识别 · 计算机科学 2021-03-15 Haoyin Zhou , Jagadeesan Jayender

Recently, super-resolution ultrasound imaging with ultrasound localization microscopy (ULM) has received much attention. However, ULM relies on low concentrations of microbubbles in the blood vessels, ultimately resulting in long…

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

图像与视频处理 · 电气工程与系统科学 2024-03-26 Yair Ben Sahel , Yonina C. Eldar