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Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be…

定量方法 · 定量生物学 2018-01-03 Paul Müller , Mirjam Schürmann , Chii J. Chan , Jochen Guck

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

Herein is presented an optical diffraction tomography (ODT) technique for measuring 3-D refractive index (RI) maps of optical plastic lenses. A Mach-Zehnder interferometer was used to measure multiple complex optical fields of a plastic…

光学 · 物理学 2016-04-20 Kyoohyun Kim , Jonghee Yoon , YongKeun Park

We present a novel illumination control technique for optical diffraction tomography (ODT). Various spatial frequencies of beam illumination were controlled by displaying time-averaged sinusoidal patterns using a digital micromirror device…

生物物理 · 物理学 2017-04-05 Kyeoreh Lee , Kyoohyun Kim , Geon Kim , Seungwoo Shin , YongKeun Park

Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has the limited access to…

光学 · 物理学 2017-11-22 Moosung Lee , Seungwoo Shin , YongKeun Park

Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral…

Optical Diffraction Tomography (ODT) is a powerful non-invasive imaging technique widely used in biological and medical applications. While significant progress has been made in transmission configuration, reflection ODT remains challenging…

光学 · 物理学 2026-05-14 Thomas Wasik , Victor Barolle , Alexandre Aubry , Josselin Garnier

Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials…

Optical diffraction tomography (ODT) is a three-dimensional (3D) quantitative phase imaging technique, which enables the reconstruction of the 3D refractive index (RI) distribution of a transparent sample. Due to its fast, non-invasive, and…

光学 · 物理学 2018-11-14 Chansuk Park , Seungwoo Shin , Yongkeun Park

Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric…

图像与视频处理 · 电气工程与系统科学 2024-12-13 Shwetadwip Chowdhury , Michael Chen , Regina Eckert , David Ren , Fan Wu , Nicole Repina , Laura Waller

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

图像与视频处理 · 电气工程与系统科学 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

光学 · 物理学 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Three-dimensional imaging of biological cells is crucial for the investigation of cell biology, provide valuable information to reveal the mechanisms behind pathophysiology of cells and tissues. Recent advances in optical diffraction…

生物物理 · 物理学 2016-03-03 Kyoohyun Kim , Jonghee Yoon , Seungwoo Shin , SangYun Lee , Su-A Yang , YongKeun Park

Optical diffraction tomography (ODT) is a powerful technique for quantitative, label-free reconstruction of the three-dimensional refractive index (RI) distribution of biological samples. While ODT is well established for imaging thin,…

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

光学 · 物理学 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park

Optical diffraction tomography (ODT) is an emerging 3D imaging technique that is used for the 3D reconstruction of the refractive index (RI) for semi-transparent samples. Various inverse models have been proposed to reconstruct the 3D RI…

光学 · 物理学 2022-06-13 Ahmed B. Ayoub , Amirhossein Saba , Carlo Gigli , Demetri Psaltis

We propose and experimentally demonstrate temporally low-coherent optical diffraction tomography (ODT) based on angle-scanning Mach-Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, we successfully…

光学 · 物理学 2018-07-17 KyeoReh Lee , Seungwoo Shin , Zahid Yaqoob , Peter T. C. So , YongKeun Park

Optical diffraction tomography (ODT), initially described in the seminal paper of Emil Wolf [Opt. Comm., 1(4), 153-156 (1969)], has received renewed attention recently. The vast majority of ODT experiments reported to date have been 3D…

Optical diffraction tomography (ODT) has emerged as an important label-free tool in biomedicine to measure the three-dimensional (3D) structure of a biological sample. In this paper, we describe ODT using second-harmonic generation (SHG)…

光学 · 物理学 2024-05-21 Amirhossein Saba , Carlo Gigli , Ye Pu , Demetri Psaltis

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI).…

定量方法 · 定量生物学 2017-08-14 Mirjam Schürmann , Gheorghe Cojoc , Salvatore Girardo , Elke Ulbricht , Jochen Guck , Paul Müller
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