相关论文: Developing a new biophysical tool to combine magne…
Biopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles. The dynamic structures…
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…
A novel non-invasive microscopy technique for imaging and sizing of folded DNA molecules with the use of photovoltaic tweezers and phase-sensitive detection is elaborated and realized. This novel method is compared with the state-of-the-art…
Combining optical tweezers with fluorescence microscopy is a powerful tool for single-cell analysis, playing a pivotal role in disease diagnosis, cell sorting, and the investigation of cellular dynamics. However, fluorescence detection…
Force spectroscopy using magnetic tweezers (MT) is a powerful method to probe the physical characteristics of single polymers. Typically, molecules are functionalized for specific attachment to a glass surface at one end and a micron-scale…
Fully internal and motional state controlled and individually manipulable polar molecules are desirable for many quantum science applications leveraging the rich state space and intrinsic interactions of molecules. While prior efforts at…
Precise control of particle positioning is desirable in many optical propulsion and sorting applications. Here, we develop an integrated platform for particle manipulation consisting of a combined optical nanofiber and optical tweezers…
Precise control of particle positioning is desirable in many optical propulsion and sorting applications. Here, we develop an integrated platform for particle manipulation consisting of a combined optical nanofibre and optical tweezers…
We report on a novel bichromatic fluorescent imaging scheme for background-free detection of single CaF molecules trapped in an optical tweezer array. By collecting fluorescence on one optical transition while using another for…
Optical tweezers, formed by tightly focused propagating laser beams, offer the unique capability to trap and control microscopic particles over a broad size range. However, the diffraction inherent to propagating optical fields, limits the…
A spatial light modulator (SLM) and a pair of galvanometer-mounted mirrors (GMM) were combined into an optical tweezers set-up. This provides great flexibility as the SLM creates an array of traps which can be moved smoothly and quickly…
Optical and optoelectronic techniques for micro- and nano-object manipulation are becoming essential tools in nano- and bio-technology. A remarkable optoelectronic technique that has experimented a strong development in the last few years…
Recent progress in quantum technologies with ultracold atoms has been propelled by spatially fine-tuned control of lasers and diffraction-limited imaging. The state-of-the-art precision of optical alignment to achieve this fine-tuning is…
As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating dye YOYO-1 against minor groove binding dye SYTO-13, using a…
Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…
We present a novel platform of optical tweezers which combines rapid prototyping of user-definable microlens arrays with spatial light modulation (SLM) for dynamical control of each associated tweezer spot. Applying femtosecond direct laser…
We study different configurations of permanent magnets and ferromagnetic circuit, in order to optimize the magnetic field for the so-called ``magnetic tweezers'' technique, for studing mechanical properties of DNA molecules. The magnetic…
Plasmonic optical tweezers are a ubiquitous tool for the precise manipulation of nanoparticles and biomolecules at low photon flux, while femtosecond-laser optical tweezers can probe the nonlinear optical properties of the trapped species…
Biophysical force spectroscopy tools - for example optical tweezers, magnetic tweezers, atomic force microscopy, - have been used to study elastic, mechanical, conformational and dynamic properties of single biological specimens from single…
Vortex-based single-beam tweezers have the ability to precisely and selectively move a wide range of objects, including particles, bubbles, droplets, and cells with sizes ranging from the millimeter to micrometer scale. In 2017, Karlsen and…