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High-speed digital holography reveals local blood flow contrasts in the eye fundus through deterministic signal analysis, leveraging a forward scattering model of dynamically diffused light. This approach enables the estimation of absolute…

仪器与探测器 · 物理学 2025-05-14 Zacharie Auray , Yann Fischer , Olivier Martinache , Michael Atlan

This work introduces a co-capture system for multi-animal visual data acquisition using conventional cameras and event cameras. Event cameras offer multiple advantages over frame-based cameras, such as a high temporal resolution and…

计算机视觉与模式识别 · 计算机科学 2022-11-23 Friedhelm Hamann , Guillermo Gallego

Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz),…

Fish embryo models are increasingly being used both for the assessment of chemicals efficacy and potential toxicity. This article proposes a methodology to automatically detect the swim bladder on 2D images of Medaka fish embryos seen…

计算机视觉与模式识别 · 计算机科学 2019-02-19 Diane Genest , Marc Léonard , Jean Cousty , Noémie De Crozé , Hugues Talbot

Laser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry…

医学物理 · 物理学 2021-08-10 Léo Puyo , Michel Paques , Michael Atlan

The performance of light-field microscopy is improved by selectively illuminating the relevant subvolume of the specimen with a second objective lens [1-3]. Here we advance this approach to a single-objective geometry, using an oblique…

Non-extractive fish abundance estimation with the aid of visual analysis has drawn increasing attention. Unstable illumination, ubiquitous noise and low frame rate video capturing in the underwater environment, however, make conventional…

计算机视觉与模式识别 · 计算机科学 2015-04-16 Meng-Che Chuang , Jenq-Neng Hwang , Kresimir Williams , Richard Towler

We report a pilot study with a wide-field laser Doppler detection scheme used to perform laser Doppler anemometry and imaging of particle seeded microflow. The optical field carrying the local scatterers (particles) dynamic state, as a…

光学 · 物理学 2013-12-24 Michel Gross , Michael Atlan , Jacques Leng

A comprehensive assessment of retinal health demands reliable and precise methods to measure localized blood perfusion. Despite considerable advancements in imaging techniques, such as indocyanine green and fluorescein angiography, along…

医学物理 · 物理学 2024-03-15 Michael Atlan

Medical ultrasound (US) is widely used to evaluate and stage vascular diseases, in particular for the preliminary screening program, due to the advantage of being radiation-free. However, automatic segmentation of small tubular structures…

计算机视觉与模式识别 · 计算机科学 2023-05-17 Zhongliang Jiang , Felix Duelmer , Nassir Navab

For decades since the development of phase-contrast optical microscopy, an analogous approach has been sought for maximizing the image contrast of weakly-scattering objects in transmission electron microscopy (TEM). The recent development…

The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome…

医学物理 · 物理学 2018-09-18 L. Puyo , M. Paques , M. Fink , J. -A. Sahel , M. Atlan

Examination of the umbilical artery with Doppler ultrasonography is performed to investigate blood supply to the fetus through the umbilical cord, which is vital for the monitoring of fetal health. Such examination involves several steps…

Fast confocal imaging was achieved by combining remote focusing with differential spinning disk optical sectioning to rapidly acquire images of live samples at cellular resolution. Axial and lateral full width half maxima less than 5 um and…

I review the method of Doppler tomography which translates binary-star line profiles taken at a series of orbital phases into a distribution of emission over the binary. I begin with a discussion of the basic principles behind Doppler…

天体物理学 · 物理学 2007-05-23 T. R. Marsh

Vascular networks can provide invaluable information about tumor angiogenesis. Ultrafast Doppler imaging enables ultrasound to image micro-vessels by applying tissue clutter filtering methods on the Spatio-temporal data obtained from…

医学物理 · 物理学 2021-01-08 Saba Adabi , Siavash Ghavami , Mostafa Fatemi , Azra Alizad

Live fish recognition is one of the most crucial elements of fisheries survey applications where vast amount of data are rapidly acquired. Different from general scenarios, challenges to underwater image recognition are posted by poor image…

计算机视觉与模式识别 · 计算机科学 2016-03-08 Meng-Che Chuang , Jenq-Neng Hwang , Kresimir Williams

In this work we present a novel publicly available stereo based 3D RGB dataset for multi-object zebrafish tracking, called 3D-ZeF. Zebrafish is an increasingly popular model organism used for studying neurological disorders, drug addiction,…

计算机视觉与模式识别 · 计算机科学 2020-06-16 Malte Pedersen , Joakim Bruslund Haurum , Stefan Hein Bengtson , Thomas B. Moeslund

Light-sheet fluorescence microscopy (LSFM) makes use of a thin plane of light to optically section and image transparent tissues or organisms {\it{in vivo}}, which has the advantages of fast imaging speed and low phototoxicity. In this…

生物物理 · 物理学 2022-09-12 Hong Ye , Xin Xu , Jixiang Wang , Jing Wang , Yi He , Yu Mu , Guohua Shi

A lateral shear interferometer based digital holographic microscopy has been realized to study the morphology dynamics of Human red blood cells quantitatively. Here, a lateral shear interferometer is embedded with a conventional microscope…

图像与视频处理 · 电气工程与系统科学 2019-09-10 Shital Devinder , Ashish Lal , Tathagato Rai Dastidar , Satish Kumar Dubey