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The flavin reductase ActVB is involved in the last step of actinorhodin biosynthesis in Streptomyces coelicolor. Although ActVB can be isolated with some FMN bound, this form was not involved in the flavin reductase activity. By studying…

生物大分子 · 定量生物学 2015-01-08 Laurent Filisetti , Julien Valton , Marc Fontecave , Vincent Nivière

This submission has been withdrawn by arXiv administration due to complaints of misuse of institutional affiliation.

生物大分子 · 定量生物学 2007-05-23 Kelath Murali Manoj

The NAD(P)H:flavin oxidoreductase from Escherichia coli, named Fre, is a monomer of 26.2 kDa that catalyzes the reduction of free flavins using NADPH or NADH as electron donor. The enzyme does not contain any prosthetic group but…

生物大分子 · 定量生物学 2015-01-08 V. Nivière , F. Fieschi , J. L. Dećout , M. Fontecave

Flavin reductase catalyzes the reduction of free flavins by NAD(P)H. As isolated, Escherichia coli flavin reductase does not contain any flavin prosthetic group but accommodates both the reduced pyridine nucleotide and the flavin substrate…

生物大分子 · 定量生物学 2014-12-17 V Nivière , M. A. Vanoni , G. Zanetti , M. Fontecave

Reducible oxides are widely used catalyst supports that can increase oxidation reaction rates by transferring their lattice oxygen at the metal-support interface. The interfacial oxidation process is typically described in terms of a…

材料科学 · 物理学 2021-04-05 Joshua L. Vincent , Peter A. Crozier

F1-ATPase is the soluble portion of the membrane-embedded enzyme FoF1-ATP synthase that catalyzes the production of adenosine triphosphate in eukaryotic and eubacterial cells. In reverse, the F1 part can also hydrolyze ATP quickly at three…

生物大分子 · 定量生物学 2015-06-18 Samuel D. Bockenhauer , Thomas M. Duncan , W. E. Moerner , Michael Boersch

Dynamic cooperativity in monomeric enzymes is characterized in terms of a non-Michaelis-Menten kinetic behaviour. The latter is believed to be associated with mechanisms that include multiple reaction pathways due to enzymatic…

化学物理 · 物理学 2016-09-21 Ashutosh Kumar , Sambarta Chatterjee , Mintu Nandi , Arti Dua

We present femtosecond transient absorption measurements on $\pi$-conjugated supramolecular assemblies in a high pump fluence regime. Oligo(\emph{p}-phenylenevinylene) monofunctionalized with ureido-\emph{s}-triazine (MOPV) self-assembles…

Flavins are the chromophores in several blue-light-sensitive photoreceptor proteins and act as redox cofactors in many enzymes relevant for biological processes. Despite their biological relevance and numerous, detailed optical…

Cell shape changes, essential for processes such as motility or division, are controlled by the actomyosin cortex that actively remodels biological membranes. Their mechanisms can be deciphered in___vitro using biomimetic reconstituted…

We developed a novel detection method for osteopontin (OPN), a new biomarker for prostate cancer, by attaching a genetically engineered single chain variable fragment (scFv) protein with high binding affinity for OPN to a carbon nanotube…

The primary step in the elusive ability of migratory birds to sense weak Earth-strength magnetic fields is supposedly the light-induced formation of a long-lived, magnetically sensitive radical pair inside a cryptochrome flavoprotein…

This thesis is focused in the study of the evolution of the metabolic repertoire of Streptomyces, which are renowned as proficient producers of bioactive Natural Products (NPs). The main goal of my work was to contribute into the…

基因组学 · 定量生物学 2022-05-31 Pablo Cruz-Morales

FoF1-ATP synthases are ubiquitous membrane-bound, rotary motor enzymes that can catalyze ATP synthesis and hydrolysis. Their enzyme kinetics are controlled by internal subunit rotation, by substrate and product concentrations, by mechanical…

生物大分子 · 定量生物学 2021-06-29 Thomas Heitkamp , Michael Börsch

In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. LB126 were investigated. The ? and ? subunits of a dioxygenase complex (FlnA1A2), showing 63% and 51% sequence identity respectively, with the subunits…

FoF1-ATP synthase catalyzes the synthesis of adenosine triphosphate (ATP). The F1 portion can be stripped from the membrane-embedded Fo portion of the enzyme. F1 acts as an ATP hydrolyzing enzyme, and ATP hydrolysis is associated with…

生物大分子 · 定量生物学 2018-02-14 Hendrik Sielaff , Thomas Heitkamp , Andrea Zappe , Nawid Zarrabi , Michael Boersch

FoF1-ATP synthase is the membrane protein catalyzing the synthesis of the 'biological energy currency' adenosine triphosphate (ATP). The enzyme uses internal subunit rotation for the mechanochemical conversion of a proton motive force to…

生物大分子 · 定量生物学 2015-06-15 Thomas Heitkamp , Hendrik Sielaff , Anja Korn , Marc Renz , Nawid Zarrabi , Michael Boersch

Multiplexed, real-time fluorescence detection at the single-molecule level is highly desirable to reveal the stoichiometry, dynamics, and interactions of individual molecular species within complex systems. However, traditionally…

We present ab initio GW plus cumulant-expansion calculations for an organic compound (TMTSF)2PF6 and a transition-metal oxide SrVO3. These materials exhibit characteristic low-energy band structures around the Fermi level, which bring about…

强关联电子 · 物理学 2016-03-02 Kazuma Nakamura , Yoshiro Nohara , Yoshihide Yoshimoto , Yusuke Nomura

We demonstrate that blending fluorinated molecules in PEDOT:PSS hole transport layers (HTL) induces charge transfers which impact on both charge extraction and photogeneration within organic photovoltaic (OPV) devices. OPVs fabricated with…

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