相关论文: Confocal supercritical angle fluorescence microsco…
3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…
The diffraction limit is a fundamental barrier in optical microscopy, which restricts the smallest resolvable feature size of a microscopic system. Microsphere-based microscopy has proven to be a promosing tool for challenging the…
The resolution of far-field optical microscopes, which rely on propagating optical modes, is widely believed to be limited because of diffraction to a value on the order of a half-wavelength $\lambda /2$ of the light used. Although…
High-contrast imaging provided by a coronagraph is critical for the direction imaging of the Earth-like planet orbiting its bright parent star. A major limitation for such direct imaging is the speckle noise that is induced from the…
Spectroscopic photoemission microscopy is a well-established method to investigate the electronic structure of surfaces. In modern photoemission microscopes the electron optics allows imaging of the image plane, momentum plane, or…
An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…
Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and…
We demonstrate a Fourier Imaging system with centimeter field of view(FOV), and 0.0042{\deg}angular resolution at C band, for the entire 180{\deg} radiation angular range. To pursue omnidirectional Fourier imaging without aberration, a…
The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…
Optical coherence tomography (OCT) microscope (OCM) uses a high-numerical-aperture objective to achieve cellular-level lateral resolution. However, its practical imaging depth range is limited by the depth of focus (DOF). Although…
Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…
I propose a superoscillation measurement method for subdiffraction incoherent optical sources, with potential applications in astronomy, remote sensing, fluorescence microscopy, and spectroscopy. The proposal, based on coherent optical…
We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…
One-shot full-field surface profilometry using digital diffractive-confocal imaging correlation microscope based on digital micromirror device is developed for one-shot microscopic 3D surface measurement. Optical configuration applies…
Imaging with a layered superlens is a spatial filtering operation characterized by the point spread function (PSF). We show that in the same optical system the image of a narrow sub-wavelength Gaussian incident field may be surprisingly…
The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…
High-resolution optical microscopy suffers from a low contrast in scattering media where a multiply scattered wave obscures a ballistic wave used for image formation. To extend the imaging depth, various gating operations - confocal,…
Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…
To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…