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The structure and function of a protein are determined by its amino acid sequence. While random mutations change a protein's sequence, evolutionary forces shape its structural fold and biological activity. Studies have shown that neutral…

生物大分子 · 定量生物学 2024-11-15 Pranav Kantroo , Günter P. Wagner , Benjamin B. Machta

The prediction of protein stability changes following single-point mutations plays a pivotal role in computational biology, particularly in areas like drug discovery, enzyme reengineering, and genetic disease analysis. Although…

定量方法 · 定量生物学 2025-05-01 Ivan Rossi , Guido Barducci , Tiziana Sanavia , Paola Turina , Emidio Capriotti , Piero Fariselli

Modern high-throughput sequencing assays efficiently capture not only gene expression and different levels of gene regulation but also a multitude of genome variants. Focused analysis of alternative alleles of variable sites at homologous…

Machine learning (ML) systems for medical imaging have demonstrated remarkable diagnostic capabilities, but their susceptibility to biases poses significant risks, since biases may negatively impact generalization performance. In this…

机器学习 · 计算机科学 2025-04-29 Haroui Ma , Francesco Quinzan , Theresa Willem , Stefan Bauer

An exactly solvable model based on the topology of a protein native state is applied to identify bottlenecks and key-sites for the folding of HIV-1 Protease. The predicted sites are found to correlate well with clinical data on resistance…

统计力学 · 物理学 2007-05-23 Cristian Micheletti , Fabio Cecconi , Alessandro Flammini , Amos Maritan

In computational molecular biology, gene regulatory binding sites prediction in whole genome remains a challenge for the researchers. Now a days, the genome wide regulatory binding site prediction tools required either direct pattern…

基因组学 · 定量生物学 2010-02-06 Chandra Prakash Singh , Feroz Khan , Sanjay Kumar Singh , Durg Singh Chauhan

We propose HSMUCE (heterogeneous simultaneous multiscale change-point estimator) for the detection of multiple change-points of the signal in a heterogeneous gaussian regression model. A piecewise constant function is estimated by…

统计方法学 · 统计学 2016-02-08 Florian Pein , Hannes Sieling , Axel Munk

Missense variant interpretation remains challenging because pathogenicity depends on heterogeneous evidence from population frequency, evolutionary conservation, transcript context, amino acid substitution severity, prior pathogenicity…

基因组学 · 定量生物学 2026-05-26 Muhammad Muneeb , David B. Ascher

Mutational signatures are patterns of somatic mutations in tumor genomes that provide insights into underlying mutagenic processes and cancer origin. Developing reliable methods for their estimation is of growing importance in cancer…

应用统计 · 统计学 2025-02-04 Blake Hansen , Isabella N. Grabski , Giovanni Parmigiani , Roberta De Vito

Proteins are responsible for the most diverse set of functions in biology. The ability to extract information from protein sequences and to predict the effects of mutations is extremely valuable in many domains of biology and medicine.…

定量方法 · 定量生物学 2018-01-04 Sam Sinai , Eric Kelsic , George M. Church , Martin A. Nowak

We propose a novel approach to detect the binding between proteins making use of the anomalous diffraction of natively present heavy elements inside the molecule 3D structure. In particular, we suggest considering sulfur atoms contained in…

Background: State-of-the art selection methods fail to identify weak but cumulative effects of features found in many high-dimensional omics datasets. Nevertheless, these features play an important role in certain diseases. Results: We…

Genetic mutations can disrupt protein structure, stability, and solubility, contributing to a wide range of diseases. Existing predictive models often lack interpretability and fail to integrate physical and chemical interactions critical…

定量方法 · 定量生物学 2025-10-01 JunJie Wee , Faisal Suwayyid , Mushal Zia , Hongsong Feng , Yuta Hozumi , Guo-Wei Wei

Biological diversity has evolved despite the essentially infinite complexity of protein sequence space. We present a hierarchical approach to the efficient searching of this space and quantify the evolutionary potential of our approach with…

统计力学 · 物理学 2009-10-31 Leonard D. Bogarad , Michael W. Deem

Recently, persistent homology has had tremendous success in biomolecular data analysis. It works by examining the topological relationship or connectivity of a group of atoms in a molecule at a variety of scales, then rendering a family of…

生物大分子 · 定量生物学 2019-03-27 David Bramer , Guo-Wei Wei

Quantifying the effects of amino acid mutations in proteins presents a significant challenge due to the vast combinations of residue sites and amino acid types, making experimental approaches costly and time-consuming. The Potts model has…

统计方法学 · 统计学 2025-05-22 Bingying Dai , Yinan Lin , Kejue Jia , Zhao Ren , Wen Zhou

Accurate identification of protein nucleic-acid-binding residues poses a significant challenge with important implications for various biological processes and drug design. Many typical computational methods for protein analysis rely on a…

生物大分子 · 定量生物学 2023-12-21 Linglin Jing , Sheng Xu , Yifan Wang , Yuzhe Zhou , Tao Shen , Zhigang Ji , Hui Fang , Zhen Li , Siqi Sun

Nonsense-mediated mRNA decay (NMD) is a critical post-transcriptional surveillance mechanism that degrades transcripts with premature termination codons, safeguarding transcriptome integrity and shaping disease phenotypes. However,…

基因组学 · 定量生物学 2025-02-21 Ali Saadat , Jacques Fellay

Accurate protein structural ensembles can be determined with metainference, a Bayesian inference method that integrates experimental information with prior knowledge of the system and deals with all sources of uncertainty and errors as well…

定量方法 · 定量生物学 2019-01-24 Thomas Löhr , Carlo Camilloni , Massimiliano Bonomi , Michele Vendruscolo

We introduce a model of DNA sequence evolution which can account for biases in mutation rates that depend on the identity of the neighboring bases. An analytic solution for this class of non-equilibrium models is developed by adopting…

生物物理 · 物理学 2007-05-23 Peter F. Arndt , Christopher B. Burge , Terence Hwa