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We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…

In this work we present a device specifically designed to study microbial growth with several applications related to environmental microbiology and other areas of research as astrobiology. The Automated Measuring and Cultivation device…

仪器与探测器 · 物理学 2012-10-29 X. C. Abrevaya , E. Cortón , O. Areso , P. J. D. Mauas

Bacteria sense a diverse range of environmental analytes with high sensitivity and temporal resolution. Engineering and synthetic biology approaches enabled harnessing this capability through development of whole-cell biosensors that…

Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases. Due to limitations…

The worldwide increase of antimicrobial resistance (AMR) is a serious threat to human health. To avert the spread of AMR, fast reliable diagnostics tools that facilitate optimal antibiotic stewardship are an unmet need. In this regard,…

Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then…

Early detection and identification of pathogenic bacteria such as Escherichia coli (E. coli) is an essential task for public health. The conventional culture-based methods for bacterial colony detection usually take >24 hours to get the…

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in…

定量方法 · 定量生物学 2022-06-07 Santosh Pandey , Yunsoo Park , Ankita Ankita , Gregory J. Phillips

External control of the swimming speed of `active particles' can be used to self assemble designer structures in situ on the micrometer to millimeter scale. We demonstrate such reconfigurable templated active self assembly in a fluid…

软凝聚态物质 · 物理学 2018-02-23 Jochen Arlt , Vincent A Martinez , Angela Dawson , Teuta Pilizota , Wilson C K Poon

Strategies for in-liquid micro-organism detection are crucial for the clinical and pharmaceutical industries. While Raman spectroscopy is a promising label-free technique for micro-organism detection, it remains challenging due to the weak…

Raman spectroscopy is a promising tool for microbial identification, yet its implementation in microbiology and clinical workflow is still restricted due to the accompanying additional preparation required to focus on microbial signals.…

定量方法 · 定量生物学 2026-03-19 Jeong Hee Kim , Jia Dong , Marissa Morales , Loza Tadesse

The lack of rapid antibiotic susceptibility tests adversely affects the treatment of bacterial infections and contributes to increased prevalence of multidrug resistant bacteria. Here, we describe an all-electrical approach that allows for…

应用物理 · 物理学 2020-05-06 Yichao Yang , Kalpana Gupta , Kamil L. Ekinci

Cell-based biosensors constitute a fundamental tool in biotechnology, and their relevance has greatly increased in recent years as a result of a surging demand for reduced animal testing and for high-throughput and cost-effective in vitro…

The mid-infrared region is crucial for elucidating the unique biochemical signatures of microorganisms. The MIR resonant structures turned out to facilitate exceptional performance owing to the enhance electric field confinement in the…

介观与纳米尺度物理 · 物理学 2024-11-15 Pooja Sudha , Anil kumar , Kunal Dhankar , Khalid Ansari , Sugata Hazra , Arup Samanta

We took benefit from Atomic Force Microscopy (AFM) in the force spectroscopy mode to describe the time evolution-over 24h-of the surface nanotopography and mechanical properties of the strain Staphylococcus aureus 27217 from bacterial…

The activity of growing living bacteria was investigated using real-time and in situ rheology -- in stationary and oscillatory shear. Two different strains of the human pathogen Staphylococcus aureus -- strain COL and its isogenic cell wall…

生物物理 · 物理学 2015-06-19 P. Patricio , P. L. Almeida , R. Portela , R. G. Sobral , I. R. Grilo , T. Cidade , C. R. Leal

Portable low-cost sensors and sensing systems for the identification and quantitative measurement of bacteria in field water are critical in preventing drinking water from being contaminated by bacteria. In this article, we reported the…

定量方法 · 定量生物学 2013-12-03 Jing Jiang , Xinhao Wang , Ran Chao , Yukun Ren , Chengpeng Hu , Zhida Xu , Gang Logan Liu

Monitoring plant health increasingly relies on leaf-mounted sensors that provide real-time physiological data, yet efficiently locating and sampling these sensors in complex agricultural environments remains a major challenge. We present an…

图像与视频处理 · 电气工程与系统科学 2025-12-12 Dexter Burns , Sanjeev Koppal

Hypothesis: Bacterial contamination of surfaces poses a major threat to public health. Designing effective antibacterial or self-cleaning surfaces requires understanding how bacteria-laden droplets interact with solid substrates and how…

软凝聚态物质 · 物理学 2025-10-29 Sirshendu Misra , Sudip Shyam , Priyam Chakraborty , Sushanta K. Mitra

Many bacterial species readily develop biofilms that act as a protective matrix against external challenge, e.g. from antimicrobial treatment. Therefore, biofilms are often responsible for persistent and recurring infections. Established…

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