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Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

计算机视觉与模式识别 · 计算机科学 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

We present a new interferometric imaging approach that allows for multiple-depth imaging in a single acquisition, using off-axis low-coherence holographic multiplexing. This technique enables sectioned imaging of multiple slices within a…

光学 · 物理学 2019-03-29 Lauren Wolbromsky , Nir A. Turko , Natan T. Shaked

High-speed multiplex imaging of fluorescent probes is limited by a combination of spectral resolution, sensitivity, high cost and low light throughput of detectors, and filters. In this work, we present a hyperspectral detection system…

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

光学 · 物理学 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…

光学 · 物理学 2015-06-15 Eric D. Diebold , Brandon W. Buckley , Daniel R. Gossett , Bahram Jalali

High-speed mid-infrared (MIR) videography constitutes an enabling tool to monitor and analyze various dynamics in scientific research and industrial applications, such as combustion diagnostics, explosion reactions, photosynthetic tracking,…

光学 · 物理学 2026-05-28 Ruiyang Qin , Kun Huang , Min Peng , Jianan Fang , Ben Sun , Zhengru Guo , Heping Zeng

Because image sensor chips have a finite bandwidth with which to read out pixels, recording video typically requires a trade-off between frame rate and pixel count. Compressed sensing techniques can circumvent this trade-off by assuming…

图像与视频处理 · 电气工程与系统科学 2019-05-31 Nick Antipa , Patrick Oare , Emrah Bostan , Ren Ng , Laura Waller

We introduce the concept of `hyperpixels' in which each element of a pixel filter array (suitable for CMOS image sensor integration) has a spectral transmission tailored to a target spectral component expected in application-specific…

Gigahertz (GHz) imaging using hard X-rays ($\gtrsim$ 10 keV) can be useful to high-temperature plasma experiments, as well as research using coherent photons from synchrotron radiation and X-ray free electron lasers. GHz framing rate can be…

仪器与探测器 · 物理学 2023-08-22 Zhehui Wang , C. L. Morris , J. S. Kapustinsky , K. Kwiatkowski , S. -N. Luo

Imaging across both the full transverse spatial and temporal dimensions of a scene with high precision in all three coordinates is key to applications ranging from LIDAR to fluorescence lifetime imaging. However, compromises that sacrifice,…

图像与视频处理 · 电气工程与系统科学 2021-01-12 C. Callenberg , A. Lyons , D. den Brok , A. Fatima , A. Turpin , V. Zickus , L. Machesky , J. Whitelaw , D. Faccio , M. B. Hullin

The discovery and understanding of many ultrafast dynamical processes are often invaluable. This paper is the first report to apply optical parametric amplification to implement single-shot ultrafast imaging with high space and time…

Microscopes face a trade-off between spatial resolution, field-of-view, and frame rate -- improving one of these properties typically requires sacrificing the others, due to the limited spatiotemporal throughput of the sensor. To overcome…

When imaging moving objects, single-pixel imaging produces motion blur. This paper proposes a new single-pixel imaging method, which can achieve anti-motion blur imaging of a fast-moving object. The geometric moment patterns and Hadamard…

图像与视频处理 · 电气工程与系统科学 2022-08-17 Zijun Guo , Wenwen Meng , Dongfeng Shi , Linbin Zha , Wei Yang , Jian Huang , Yafeng Chen , Yingjian Wang

Through pixel-wise optical coding of images during exposure time, it is possible to extract sub-exposure images from a single capture. Such a capability can be used for different purposes, including high-speed imaging, high-dynamic-range…

计算机视觉与模式识别 · 计算机科学 2018-02-14 Shah Rez Khan , Martin Feldman , Bahadir K. Gunturk

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

光学 · 物理学 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

This paper introduces a single-pixel HyperSpectral (HS) imaging framework based on Fourier Transform Interferometry (FTI). By combining a space-time coding of the light illumination with partial interferometric observations of a collimated…

计算机视觉与模式识别 · 计算机科学 2018-09-05 Amirafshar Moshtaghpour , José M. Bioucas-Dias , Laurent Jacques

We report a demonstration of laser Doppler holography at a sustained acquisition rate of 250 Hz on a 1 Megapixel complementary metal-oxide-semiconductor (CMOS) sensor array and image display at 10 Hz frame rate. The holograms are optically…

光学 · 物理学 2015-06-12 Benjamin Samson , Michael Atlan

Sonography techniques use multiple transducer elements for tissue visualization. Signals detected at each element are sampled prior to digital beamforming. The required sampling rates are up to 4 times the Nyquist rate of the signal and…

信息论 · 计算机科学 2012-12-21 T. Chernyakova , Y. C. Eldar , R. Amit

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Sonography techniques use multiple transducer elements for tissue visualization. Signals detected at each element are sampled prior to digital beamforming. The sampling rates required to perform high resolution digital beamforming are…

信息论 · 计算机科学 2013-07-25 Tanya Chernyakova , Yonina C. Eldar
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