相关论文: Measuring, in solution, multiple-fluorophore label…
The angular distribution and linear polarization of the fluorescence light following the resonant photoexcitation is investigated within the framework of the density matrix and second-order perturbation theory. Emphasis has been placed on…
The investigation of the static and dynamic structural properties of colloidal systems relies on techniques capable of atomic resolution in real space and femtosecond resolution in time. Recently, the cross-correlation function (CCF)…
We describe a method to probe the spectral fluctuations of a transition over broad ranges of frequencies and timescales with the high spectral resolution of Fourier spectroscopy, and a temporal resolution as high as the excited state…
A multispeckle technique for efficiently measuring correctly ensemble-averaged intensity autocorrelation functions of scattered light from non-ergodic and/or non-stationary systems is described. The method employs a CCD camera as a…
Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…
Extracellular vesicles (EVs) have drawn rapidly increasing attention as the next-generation diagnostic biomarkers and therapeutic agents. However, the heterogeneous nature of EVs necessitates advanced methods for profiling EVs at the…
Amphiphilic molecules such as surfactants, lipids and block copolymers can be assembled into bilayers and form vesicles. Fluorescent membrane labeling methods require the use of dye molecules that can be inserted in the bilayers at…
Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…
A novel non-invasive microscopy technique for imaging and sizing of folded DNA molecules with the use of photovoltaic tweezers and phase-sensitive detection is elaborated and realized. This novel method is compared with the state-of-the-art…
Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…
For the assessment of radiation effects of clinical ion-beams, dosimetry has to be complemented by information on particle-energy distribution or related quantities. Fluorescence nuclear track detectors made from C,Mg-doped alumina single…
Fusion energy research increasingly depends on the ability to integrate heterogeneous, multimodal datasets from high-resolution diagnostics, control systems, and multiscale simulations. The sheer volume and complexity of these datasets…
A commonly held tenet is that lasers well above threshold emit photons in a coherent state, which follow a Poissonian statistics when measured in photon number. This feature is often exploited to build quantum-based random number generators…
Flow cytometry is a technique that measures multiple fluorescence and light scatter-associated parameters from individual cells as they flow a single file through an excitation light source. These cells are labeled with antibodies to detect…
We have performed single molecule stretching experiments and dynamic light scattering (DLS) in order to characterize the interaction between the DNA molecule and the fluorescent stain GelRed. The results from single molecule stretching show…
Label-free imaging has gained broad interest because of its potential to omit elaborate staining procedures which is especially relevant for in vivo use. Label-free multiphoton microscopy (MPM), for instance, exploits two-photon excitation…
Fluorescence lifetime experiments are a standard approach for measuring excited state dynamics and local environment effects. Here, we show that entangled photon pairs produced from a continuous-wave (CW) laser diode can replicate pulsed…
Confocal fluorescence microscopy is a proven technique, which can image near-electrode pH changes. For a complete understanding of electrode processes, time-resolved measurements are required, which have not yet been provided. Here we…
As single-photon sources become more mature and are used more often in quantum information, communications and measurement applications, their characterization becomes more important. Single-photon-like light is often characterized by its…
This paper addresses the problem of quantifying biomarkers in multi-stained tissues, based on color and spatial information. A deep learning based method that can automatically localize and quantify the cells expressing biomarker(s) in a…