定量方法
Objective: Quantitative real-time PCR is widely used for gene expression analysis, yet inconsistencies in data storage and reporting limit reproducibility. While MIQE guidelines define the minimal information required for publication, they…
High-resolution spatial transcriptomics (HR-ST) technologies offer unprecedented insights into tissue architecture but lack standardized frameworks for histological annotation. We present ST2HE, a cross-platform generative framework that…
Replication of genetic material is an important process for all living organisms. Origins of replication initiate the copying of DNA at many points on a chromosome, and it is the distribution of these points that is relevant here, as it…
Mammalian tissue architecture is central to biological function, and its disruption is a hallmark of disease. Medical imaging techniques can generate large point cloud datasets that capture changes in the cellular composition of such…
Prostate cancer (PCa) remains a significant global health concern among men, particularly due to the lethality of its more aggressive variants. Despite therapeutic advancements that have enhanced survival for many patients, high grade PCa…
Conflicting clinical trial results on omega-3 highly unsaturated fatty acids (n-3 HUFA) have prompted uncertainty about their cardioprotective effects. While the VITAL trial found no overall cardiovascular benefit from n-3 HUFA…
This paper introduces the Single-Cell Perturbation Prediction Diffusion Model (scPPDM), the first diffusion-based framework for single-cell drug-response prediction from scRNA-seq data. scPPDM couples two condition channels,…
Open biochemical systems of interacting molecules are ubiquitous in life-related processes. However, established computational methodologies, like molecular dynamics, are still mostly constrained to closed systems and timescales too small…
The lack of standardization in seizure forecasting slows progress in the field and limits the clinical translation of forecasting models. In this work, we introduce a Python-based framework aimed at streamlining the development, assessment,…
Untargeted metabolomics using LC-MS/MS offers the potential to comprehensively profile the chemical diversity of biological samples. However, the process is fundamentally limited by the "identification bottleneck," where only a small…
European grapevine (\textit{Vitis vinifera} L.) is a climate-sensitive perennial whose flowering and ripening govern yield and quality. Phenological records from monitoring programs are typically collected at irregular intervals, so true…
Biological processes rely on finely tuned homo- and heteromeric interactions between (biomacro)molecules. The strength of an interaction, typically given by the dissociation constant (KD), plays a crucial role in basic research and must be…
We introduce AbBiBench (Antibody Binding Benchmarking), a benchmarking framework for antibody binding affinity maturation and design. Unlike previous strategies that evaluate antibodies in isolation, typically by comparing them to natural…
Persistent homology applied to the activity of grid cells in the Medial Entorhinal Cortex suggests that this activity lies on a toroidal manifold. By analyzing real data and a simple model, we show that neural oscillations play a key role…
Simulating in silico cellular responses to interventions is a promising direction to accelerate high-content image-based assays, critical for advancing drug discovery and gene editing. To support this, we introduce MorphGen, a…
Early recurrence (ER) prediction after curative-intent resection remains a critical challenge in the clinical management of hepatocellular carcinoma (HCC). Although contrast-enhanced computed tomography (CT) with full multi-phase…
Surgical video datasets are essential for scene understanding, enabling procedural modeling and intra-operative support. However, these datasets are often heavily imbalanced, with rare actions and tools under-represented, which limits the…
Wuchereria bancrofti, the parasitic roundworm responsible for lymphatic filariasis, permanently disables over 36 million people and places 657 million at risk across 39 countries. A major bottleneck for drug discovery is the lack of…
Large Language Models (LLMs) and LLM-based agents show great promise in accelerating scientific research. Existing benchmarks for measuring this potential and guiding future development continue to evolve from pure recall and rote knowledge…
Protein sequence alignment is a cornerstone of bioinformatics, traditionally approached using dynamic programming (DP) algorithms that find an optimal sequential path. This paper introduces UniOTalign, a novel framework that recasts…