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3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

We aim to clarify the role that absorption plays in nonlinear optical processes in a variety of metallic nanostructures and show how it relates to emission and conversion efficiency. We define a figure of merit that establishes the…

Entangled-photon coincidence imaging is a method to nonlocally image an object by transmitting a pair of entangled photons through the object and a reference optical system, respectively. The image of the object can be extracted from the…

Quantum Physics · Physics 2016-09-08 Jing Cheng , Shensheng Han

We use femtosecond resonant soft x-ray diffraction to measure the optically stimulated ultrafast changes of charge density wave correlations in underdoped YBa2Cu3O6.6. We find that when coherent interlayer transport is enhanced by optical…

Extreme intensity contrast is considered essential for ultraintense, femtosecond laser excitation of solid targets, in particular for studies with structured or ultra-thin targets. Second-harmonic generation has been used to maximize the…

Quantum imaging is an advanced method for microscopy or investigating the optical properties of materials or bio-medical inspections with high accuracy, low noise, and extremely low photo-damage. In previous work, we proposed a quantum…

Optics · Physics 2025-05-09 Mousume Samad , Maki Shimizu , Yasuto Hijikata

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

In this work, we present the implementation of all-optical method for directly measuring electron pulse duration in an ultrafast scanning electron microscope. Our approach is based on the interaction of electrons with the ponderomotive…

Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent…

Quantitative Methods · Quantitative Biology 2009-11-10 R. V. Krishnan , A. Masuda , V. E. Centoze , B. Herman

The use of individual multimode optical fibers in endoscopy applications has the potential to provide highly miniaturized and noninvasive probes for microscopy and optical micromanipulation. A few different strategies have been proposed…

Harnessing nonlinear optical effects in a photonic chip scale has been proven useful for a number of key applications in optical communications. Microwave photonics can also benefit from the adoption of such a technology, creating a new…

Optics · Physics 2015-06-17 David Marpaung , Benjamin J. Eggleton

Mid-infrared photothermal microscopy is a highly promising imaging technique that enables spatially resolved vibrational fingerprinting. The combination of infrared induced heating with optical readout at visible wavelengths provides…

Optics · Physics 2025-08-05 Dennis van de Lockand , Daan Wolters , Matz Liebel

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

Optics · Physics 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

We demonstrate orientation-sensitive multimodal nonlinear optical polarizing microscopy capable of probing orientational, polar, and biaxial features of mesomorphic ordering in soft matter. This technique achieves simultaneous imaging in…

Soft Condensed Matter · Physics 2015-05-19 Taewoo Lee , Rahul P. Trivedi , Ivan I. Smalyukh

An important topic of interest in imaging is the construction of protocols that are not diffraction limited. This can be achieved in a variety of ways, including classical superresolution techniques or quantum entanglement-based protocols.…

Quantum Physics · Physics 2015-10-28 Mark E. Pearce , Thomas Mehringer , J. von Zanthier , Pieter Kok

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…

Biological Physics · Physics 2023-10-27 Pavel Malý , Dita Strachotová , Aleš Holoubek , Petr Heřman

Over the past few years, the combination of diffuser and near-field electron ptychography has drawn more attention by its ability to recover large field of view with few diffraction patterns. In this paper, we purpose a novel design and…

Extracting as much information as possible about an object when probing with a limited number of photons is an important goal with applications from biology and security to metrology. Imaging with a few photons is a challenging task as the…