Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera
Quantitative Methods
2009-11-10 v1 Cell Behavior
Abstract
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent developments in multiphoton FLIM methods and also present a description of a novel multiphoton FLIM system using a streak camera that was developed in our laboratory. We provide an example of a typical application of the system in which we measure the fluorescence resonance energy transfer between a donor/acceptor pair of fluorescent proteins within a cellular specimen.
Keywords
Cite
@article{arxiv.q-bio/0309022,
title = {Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera},
author = {R. V. Krishnan and A. Masuda and V. E. Centoze and B. Herman},
journal= {arXiv preprint arXiv:q-bio/0309022},
year = {2009}
}
Comments
Overview of FLIM techniques, StreakFLIM instrument, FRET applications