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We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…

Today's diagnostics include devices such as pulse oximeters, blood pressure monitors, and temperature measurements. These devices provide vital information to medical personnel when making treatment decisions. Drawing inspiration from the…

Image and Video Processing · Electrical Eng. & Systems 2022-08-26 Akhil Kallepalli , David B James , Mark A Richardson

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

Image simulation remains under-exploited for the most widely used biological phase microscopy methods, because of difficulties in simulating partially coherent illumination. We describe an open-source toolbox, microlith…

Optics · Physics 2014-05-14 Shalin B. Mehta , Rudolf Oldenbourg

Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

Spatial modulation microscopy is a technique originally developed for quantitative spectroscopy of individual nano-objects. Here, a parallel implementation of the spatial modulation microscopy technique is demonstrated based on a line…

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

Optics · Physics 2025-01-22 Jaromír Běhal , Miroslav Ježek

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

A recently identified class of receivers which demultiplex an optical field into a set of orthogonal spatial modes prior to detection can surpass canonical diffraction limits on spatial resolution for simple incoherent imaging tasks.…

Quantum Physics · Physics 2021-02-05 Michael R Grace , Zachary Dutton , Amit Ashok , Saikat Guha

Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural…

A low cost remote imaging platform for biological applications was developed. The "Picroscope" is a device that allows the user to perform longitudinal imaging studies on multi-well cell culture plates. Here we present the network…

This paper considers the problem of sampling and reconstruction of a continuous-time sparse signal without assuming the knowledge of the sampling instants or the sampling rate. This topic has its roots in the problem of recovering multiple…

Information Theory · Computer Science 2017-01-31 Ayush Bhandari , Aurelien Bourquard , Ramesh Raskar

A technique to simulate the spex-mixer/mill system as a macroscopic ensemble rather than a pure dynamical system is proposed. The treatment is suitable especially for comminution processes generating the nanomaterial up to nanometers scale…

Computational Physics · Physics 2010-07-08 Muhandis , F. Nurdiana , A. S. Wismogroho , N. T. Rochman , L. T. Handoko

The automated segmentation and tracking of macrophages during their migration are challenging tasks due to their dynamically changing shapes and motions. This paper proposes a new algorithm to achieve automatic cell tracking in time-lapse…

Image and Video Processing · Electrical Eng. & Systems 2023-01-03 Seol Ah Park , Tamara Sipka , Zuzana Kriva , George Lutfalla , Mai Nguyen-Chi , Karol Mikula

Inspection of tissues using a light microscope is the primary method of diagnosing many diseases, notably cancer. Highly multiplexed tissue imaging builds on this foundation, enabling the collection of up to 60 channels of molecular…

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous…

Optics · Physics 2024-09-04 Carlo Bevilacqua , Robert Prevedel

Ptychography is a robust lensless form of microscopy routinely used for applications spanning life and physical sciences. The most common ptychography setup consists in using a detector to record diffraction patterns in the far-field. A…

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