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Studying the growth and metabolism of microbes provides critical insights into their evolutionary adaptations to harsh environments, which are essential for microbial research and biotechnology applications. In this study, we developed an…

Image and Video Processing · Electrical Eng. & Systems 2025-05-06 Shuang Zhang , Carleton Coffin , Karyn L. Rogers , Catherine Ann Royer , Ge Wang

Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…

Optics · Physics 2024-11-20 Vahid Nasirimarekani , Zuzana Ditte , Eberhard Bodenschatz

This paper presents deformable templates as a tool for segmentation and localization of biological structures in medical images. Structures are represented by a prototype template, combined with a parametric warp mapping used to deform the…

Computer Vision and Pattern Recognition · Computer Science 2007-05-23 Jonathan M. Spiller , T. Marwala

Here a new microscopic method is proposed to image and characterize very thin samples like few-layer materials, organic molecules, and nanostructures with nanometer or sub-nanometer resolution using electron beams of energies lower than 20…

Instrumentation and Detectors · Physics 2016-01-06 Ing-Shouh Hwang

Spatial frequency filtering is a fundamental enabler of information processing methods in biological and technical imaging. Most filtering methods, however, require either bulky and expensive optical equipment or some degree of…

Reflective ptychography is a promising lensless imaging technique with a wide field of view, offering significant potential for applications in semiconductor manufacturing and detection. However, many semiconductor materials are coated with…

Optics · Physics 2025-01-03 Yun Gao , Qijun You , Peixiang Lu , Wei Cao

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

Brillouin and Raman scattering spectroscopy are established techniques for the nondestructive contactless and label-free readout of mechanical, chemical and structural properties of condensed matter. Brillouin-Raman investigations currently…

This work presents a high-resolution X-ray microtomography system that uses commercial off-the-shelf (COTS) CMOS image sensors as direct detectors, relying on the sensor s intrinsic resolution to achieve tomographic reconstructions without…

Image and Video Processing · Electrical Eng. & Systems 2026-05-29 Damian L. Corzi , Jose Lipovetzky , Fabricio Alcalde Bessia , German Mato , Andres Cicuttin , Maria L. Crespo , Martin Perez , Mariano Gomez Berisso

Achieving high spatial resolution is the goal of many imaging systems. Designing a high-resolution lens with diffraction-limited performance over a large field of view remains a difficult task in imaging system design. On the other hand,…

Extracting reliable and quantitative microstructure information of living tissue by non-invasive imaging is an outstanding challenge for understanding disease mechanisms and allowing early stage diagnosis of pathologies. Magnetic Resonance…

Medical Physics · Physics 2021-07-12 Milena Capiglioni , Analia Zwick , Pablo Jimenez , Gonzalo A. Alvarez

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However,…

Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

Imaging of biological cells and tissues often relies on fluorescent labels, which offer high contrast with molecular specificity. The use of exogenous labeling agents, however, may alter the normal physiology of the bio-specimens.…

Instrumentation and Detectors · Physics 2019-10-01 Zichao Bian , Shaowei Jiang , Pengming Song , He Zhang , Pouria Hoveida , Kazunori Hoshino , Guoan Zheng

Fourier ptychographic microscopy enables gigapixel-scale imaging, with both large field-of-view and high resolution. Using a set of low-resolution images that are recorded under varying illumination angles, the goal is to computationally…

Signal Processing · Electrical Eng. & Systems 2018-03-13 Emrah Bostan , Mahdi Soltanolkotabi , David Ren , Laura Waller

The extraordinary improvements of modern imaging devices offer access to data with unprecedented information content. However, widely used image processing methodologies fall far short of exploiting the full breadth of information offered…

Computer Vision and Pattern Recognition · Computer Science 2014-03-27 Benjamin Berkels , Peter Binev , Douglas A. Blom , Wolfgang Dahmen , Robert C. Sharpley , Thomas Vogt

Hyperspectral imaging is useful for applications ranging from medical diagnostics to agricultural crop monitoring; however, traditional scanning hyperspectral imagers are prohibitively slow and expensive for widespread adoption. Snapshot…

Image and Video Processing · Electrical Eng. & Systems 2020-09-30 Kristina Monakhova , Kyrollos Yanny , Neerja Aggarwal , Laura Waller