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Nonlinear optical microscopy techniques have emerged as a set of successful tools for biological imaging. Stimulated emission microscopy belongs to a small subset of pump-probe techniques which can image non-fluorescent samples without…

Scanning Probe Microscopy allows for extreme resolution down to the atomic scale. Unfortunately, total scanning range is rather limited, therefore finding a specific position on the sample is tedious. This is an important limitation of many…

Materials Science · Physics 2015-06-12 José Abad , Juan Francisco González Martínez , Jaime Colchero Paetz

Brillouin microscopy is an emerging optical technique for probing mechanical properties with submicron resolution, offering fully non-contact, label-free operation. Despite its unique capabilities, broader adoption has been limited by slow…

Optics · Physics 2026-04-22 Mikolaj Pochylski

Mid-InfraRed spectroscopy is a promising label-free technique that can offer insights into morphological and pathological alterations in biological tissues at the molecular level. Owing to the development of the Fourier Transform InfraRed…

Optics · Physics 2026-04-21 Xiangyu Zhao , Yudong Tian , Jingzhu Shao , Chongzhao Wu

Microtubules are filament-shaped, polymeric proteins (~25 nm in diameter) involved in cellular structure and organization. We demonstrate the imaging of individual microtubules using a conventional bright-field microscope, without any…

Biological Physics · Physics 2015-05-14 Braulio Gutiérrez-Medina , Steven M. Block

We present the experimental reconstruction of sub-wavelength features from the far-field intensity of sparse optical objects: sparsity-based sub-wavelength imaging combined with phase-retrieval. As examples, we demonstrate the recovery of…

We present a Scanning Hall Probe Microscope operating in ambient conditions. One of the unique features of this microscope is the use of the same stepper motors for both sample positioning as well as scanning, which makes it possible to…

Mesoscale and Nanoscale Physics · Physics 2017-12-12 Gorky Shaw , R. B. G. Kramer , N Dempsey , K Hasselbach

Diffraction limit is manifested in the loss of high spatial frequency information that results from decay of evanescent waves. As a result, conventional far-field optics yields no information about an object's subwavelength features. Here…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

Microscopes are vital pieces of equipment in much of biological research and medical diagnostics. However, access to a microscope can represent a bottleneck in research, especially in lower-income countries. `Smart' computer controlled…

Instrumentation and Detectors · Physics 2022-07-08 Samuel McDermott , Filip Ayazi , Joel Collins , Joe Knapper , Julian Stirling , Richard Bowman , Pietro Cicuta

This article presents a method to perform diffraction tomography in a standard microscope that includes an LED array for illumination. After acquiring a sequence of intensity-only images of a thick sample, a ptychography-based…

Optics · Physics 2015-10-30 Roarke Horstmeyer , Changhuei Yang

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon

The flexible endoscope is a minimally invasive tool in clinical settings, but most of them rely on exogenous staining for diagnosis to provide qualitative information. Here, we demonstrated a flexible endoscopic microscopy (FEM) with…

On-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and…

Optical endoscopy plays a crucial role in minimally invasive medical diagnostics and therapeutic procedures. Nonetheless, state-of-the-art endoscopic zoom objectives are bulky, requiring multi-element lens actuation, which can limit…

We describe a technique for extracting topographic information using a scanning near-field microwave microscope. By monitoring the shift of the system's resonant frequency, we obtain quantitative topographic images of uniformly conducting…

We have developed a hyperspectral imaging scheme that involves a combination of dual-comb spectroscopy and Hadamard-transform-based single-pixel imaging. The scheme enables us to obtain 12,000 hyperspectral images of amplitude and phase at…

Microscope mode imaging for secondary ion mass spectrometry is a technique with the promise of simultaneous high spatial resolution and high speed imaging of biomolecules from complex surfaces. Technological developments such as new…

Instrumentation and Detectors · Physics 2013-09-05 András Kiss , Donald F. Smith , Julia H. Jungmann , Ron M. A. Heeren

Super-resolution microscopes (such as STED) illuminate samples with a tiny spot, and achieve very high resolution. But structures smaller than the spot cannot be resolved in this way. Therefore, we propose a technique to solve this problem.…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

We present a new imaging technique, swept-angle synthetic wavelength interferometry, for full-field micron-scale 3D sensing. As in conventional synthetic wavelength interferometry, our technique uses light consisting of two…

Computer Vision and Pattern Recognition · Computer Science 2023-03-31 Alankar Kotwal , Anat Levin , Ioannis Gkioulekas

Ptychography is a promising phase retrieval technique for visible light, X-ray and electron beams. Conventional ptychography reconstructs the amplitude and phase of an object light from a set of the diffraction intensity patterns obtained…

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