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Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…
Structured illumination microscopy (SIM) can achieve a $2\times$ resolution enhancement beyond the classical diffraction limit by employing illumination translations with respect to the object. This method has also been successfully…
A method is proposed for assessing the temporal resolution of Structured Illumination Microscopy (SIM), by tracking the amplitude of different spatial frequency components over time, and comparing them to a temporally-oscillating…
Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different…
The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…
We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…
Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…
Structured illumination microscopy (SIM) can double the resolution beyond the light diffraction limit, but it comes at the cost of multiple camera exposures and the heavy computation burden of multiple Fourier transforms. In this paper, we…
By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction…
Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…
Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…
Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…
Structured Illumination Microscopy (SIM) enables rapid, high-contrast optical sectioning of fresh tissue without staining or physical sectioning, making it promising for intraoperative and point-of-care diagnostics. Recent foundation and…
In Super-resolution, a varying-illumination image stack is required. This enriched the dataset typically necessitates precise mechanical control and micron scale optical alignment and repeatability. Here, we introduce a novel methodology…
Confocal microscopy, a critical advancement in optical imaging, is widely applied because of its excellent anti-noise ability. However, it has low imaging efficiency and can cause phototoxicity. Optical-sectioning structured illumination…
Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…
In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…
For quality control in the factory, 3D-metrology faces increasing demands for high precision and for more space-bandwidth-speed-product SBSP (number of 3D-points/sec). As a potential solution, we will discuss Structured-Illumination…
Modern optical microscopy methods have been advanced to provide super resolution at high imaging speed, but not chirality discriminative. We recently proposed chiral structured-illumination microscopy (SIM) method to image chiral…