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Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

We propose to use intensity correlation microscopy in combination with structured illumination to image quantum emitters that exhibit antibunching with a spatial resolution reaching far beyond the Rayleigh limit. Combining intensity…

Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination…

Optics · Physics 2021-07-28 Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

The structured illumination microscopy using unknown speckle patterns has shown the capacity to surpass the Abbe's diffraction barrier, giving the possibility to design cheap and versatile SIM devices. However, the state-of-the-art joint…

Image and Video Processing · Electrical Eng. & Systems 2020-08-27 Penghuan Liu

We report a novel extension to structured illumination (SI) microscopy that utilizes the Talbot self-imaging effect to generate a SI pattern on a sample with field-of-view (FOV) and resolution unconstrained by the numerical aperture (NA) of…

Optics · Physics 2018-01-12 Shwetadwip Chowdhury , Jeffrey Chen , Joseph A. Izatt

Mass spectrometry imaging (MSI) as an analytical tool for bio-molecular and bio-medical research targets, accurate compound localization and identification. In terms of dedicated instrumentation, this translates into the demand for more…

Instrumentation and Detectors · Physics 2013-05-24 Julia H. Jungmann , Ron M. A. Heeren

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

Optics · Physics 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park

Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

Optics · Physics 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

Neural networks possess incredible capabilities for extracting abstract features from data. Electromagnetic computing harnesses wave propagation to execute computational operations. Metasurfaces, composed of subwavelength meta-atoms, are…

Information Theory · Computer Science 2026-01-23 Jiancheng An , Chau Yuen , Marco Di Renzo , Mehdi Bennis , Merouane Debbah , Lajos Hanzo

A resolution-enhanced dark-field microscope by structured light illumination is proposed to improve resolution and contrast. A set of phase-shifted fringes are projected to the sample plane at large angle to capture modulated dark-field…

Image and Video Processing · Electrical Eng. & Systems 2022-02-14 Shaobai Li , Bofan Song , Rongguang Liang

The revolutionary technology of \emph{Stacked Intelligent Metasurfaces (SIM)} has been recently shown to be capable of carrying out advanced signal processing directly in the native electromagnetic (EM) wave domain. An SIM is fabricated by…

Information Theory · Computer Science 2023-05-16 Jiancheng An , Chao Xu , Derrick Wing Kwan Ng , George C. Alexandropoulos , Chongwen Huang , Chau Yuen , Lajos Hanzo

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

We demonstrate the combination of a hemispherical solid immersion lens with a micro-photoluminescence setup. Two advantages introduced by the SIL, an improved resolution of 0.4 times the wavelength in vacuum and a 5 times enhancement of the…

Optics · Physics 2009-11-07 S. Moehl , Hui Zhao , B. Dal Don , S. Wachter , H. Kalt

The principles of quantum optics have yielded a plethora of ideas to surpass the classical limitations of sensitivity and resolution in optical microscopy. While some ideas have been applied in proof-of-principle experiments, imaging a…

This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…

Quantitative Methods · Quantitative Biology 2023-11-02 Aniruddha Acharya

Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

We present a simple and compact approach of structured illumination microscopy by using three $2\times2$ fiber couplers and one $1\times4$ MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven…

Optics · Physics 2019-12-18 Shiming Hu , Wenwen Liu , Junyao Jie , Yizheng Huang , Qingquan Wei , Manqing Tan , Yude Yu

Clinical MRI frequently acquires anisotropic volumes with high in-plane resolution and low through-plane resolution to reduce acquisition time. Multiple orientations are therefore acquired to provide complementary anatomical information.…

Image and Video Processing · Electrical Eng. & Systems 2026-03-25 Heejong Kim , Abhishek Thanki , Roel van Herten , Daniel Margolis , Mert R Sabuncu