High-speed image reconstruction for nonlinear structured illumination microscopy
Optics
2023-12-05 v1 Biological Physics
Abstract
By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction method of nonlinear structured illumination microscopy (NL-SIM) is relatively slow, which brings a great challenge to realizing real-time display of super-resolution results. To address these issues, an accelerated NL-SIM reconstruction algorithm was developed by extending a high-speed reconstruction framework, Joint Space and Frequency Reconstruction (JSFR) to NL-SIM. We anticipate that this algorithm will facilitate NL- SIM becoming a routine tool in biomedical laboratories.
Keywords
Cite
@article{arxiv.2312.01073,
title = {High-speed image reconstruction for nonlinear structured illumination microscopy},
author = {Jingxiang Zhang and Tianyu Zhao and Xiangda Fu and Manming Shu and Jiajing Yan and Jinxiao Chen and Yansheng Liang and Shaowei Wang and Ming Lei},
journal= {arXiv preprint arXiv:2312.01073},
year = {2023}
}