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Dark Field X-ray Microscopy (DFXM) has advanced 3D non-destructive, high-resolution imaging of strain and orientation in crystalline materials, enabling the study of embedded structures in bulk. However, the photon-intensive nature of…
Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…
A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…
Fourier ptychographic microscopy (FPM) is a pivotal computational imaging technique that achieves phase and amplitude reconstruction with high resolution and wide field of view, using low numerical aperture objectives and LED array…
Obtaining 3D information from a single X-ray exposure at high-brilliance sources, such as X-ray free-electron lasers (XFELs) [1] or diffraction-limited storage rings [2], allows the study of fast dynamical processes in their native…
Digital cameras and displays utilise picture elements (pixels) that perform a single function: detecting or emitting light intensity. To exploit the full information content of electromagnetic waves, more advanced elements are required.…
We propose a holographic microscopy method based on single-pixel imaging technology (HM-SPI). We used a holographic microscopy method based on in-line Gabor holography. In single-pixel imaging technology, cyclic binary masks and…
Extracting as much information as possible about an object when probing with a limited number of photons is an important goal with applications from biology and security to metrology. Imaging with a few photons is a challenging task as the…
We demonstrate an electro-optic wide-field method to enable fluorescence lifetime microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven Pockels cells are used to efficiently gate images at 39 MHz,…
Despite their widespread use in cell biology, fluorescence lifetime imaging microscopy (FLIM) data-sets are challenging to analyse, because each spatial position can contain a superposition of multiple fluorescent components. Here, we…
While polarisation sensing is vital in many areas of research, with applications spanning from microscopy to aerospace, traditional approaches are limited by method-related error amplification or accumulation, placing fundamental…
Tapered optical fibers (TFs), with diameters gradually reduced from hundreds of microns to the micron scale, offer key advantages over conventional flat optical fibers (FFs), including uniform illumination, efficient long-range signal…
Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…
A novel technique for polarization-multiplexing ghost imaging is proposed to simultaneously obtain multiple polarimetric information by a single detector. Here, polarization-division multiplexing speckles are employed for object…
An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…
The Fourier inversion of phased coherent diffraction patterns offers images without the resolution and depth-of-focus limitations of lens-based tomographic systems. We report on our recent experimental images inverted using recent…
We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…
In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…
Hyperspectral imaging is an important tool having been applied in various fields, but still limited in observation of dynamic scenes. In this paper, we propose a snapshot hyperspectral imaging technique which exploits both spectral and…
Femtosecond-scale ultrafast imaging is an essential tool for visualizing ultrafast dynamics in molecular biology, physical chemistry, atomic physics, and fluid dynamics. Pump-probe imaging and a streak camera are the most widely used…