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We present Full SMS, a multipurpose graphical user interface (GUI)-based software package for analysing single-molecule spectroscopy (SMS) data. SMS typically delivers multiparameter data -- such as fluorescence brightness, lifetime, and…

Biological Physics · Physics 2024-05-01 Joshua L. Botha , Bertus van Heerden , Tjaart P. J. Krüger

We present an open source software package SpectroLab a Matlab-based tool developed in 2018 for the analysis of spectroscopic data. In this package, there are tools for derivative analysis, stacked energy contours, stacked plots for theory,…

Materials Science · Physics 2022-03-15 Christopher Sims

INTEGRAL observations provide a large amount of data on accreting binary systems. The interpretation of the spectral emission of these sources needs timing analysis and phase resolved spectroscopy, which are really cumbersome tasks if…

Astrophysics · Physics 2007-09-27 A. Segreto , C. Ferrigno

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…

Signal Processing · Electrical Eng. & Systems 2023-07-19 Thanh-An Pham , Emmanuel Soubies , Ferréol Soulez , Michael Unser

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…

Quantitative Methods · Quantitative Biology 2017-10-24 C. Chen , P. Paul-Gilloteaux , T. Vignaud , J. Salamero , F. Waharte

Spectroscopy is a central pillar of materials characterization, providing useful information on properties like structure, composition, or excited state dynamics of a system. However, many spectroscopic techniques present challenges in…

Materials Science · Physics 2026-04-09 Amalya C. Johnson , Chris Fajardo , Leena Sansguiri , Weike Ye , Steven B. Torrisi

Single molecule spectroscopy aims at unveiling often hidden but potentially very important contributions of single entities to a system's ensemble response. Albeit contributing tremendously to our ever growing understanding of molecular…

Chemical Physics · Physics 2017-12-19 Matz Liebel , Costanza Toninelli , Niek F. van Hulst

Advanced microscopy techniques are essential in biomedical research for visualising and tracking biomolecules within living cells and their compartments. Conventional fluorescence microscopy methods, however, often struggle with accurately…

The possibility of using time-resolved vibronic spectroscopy for spectral analysis of mixtures of chemical compounds with similar optical properties, when traditional methods are inefficient, is demonstrated by using the method of computer…

Chemical Physics · Physics 2007-05-23 S. A. Astakhov , V. I. Baranov

Real time computer control is an essential feature of scanning probe microscopes, which have become essential tools for the characterization and investigation of nanometer scale samples. Most commercial (and some open-source) scanning probe…

Mesoscale and Nanoscale Physics · Physics 2013-01-24 Venkat Chandrasekhar , Manan Mehta

Novel multiplexing triple-axis neutron scattering spectrometers yield significant improvements of the common triple-axis instruments. While the planar scattering geometry keeps ensuring compatibility with complex sample environments, a…

Data Analysis, Statistics and Probability · Physics 2026-03-02 Jakob Lass , Henrik Jacobsen , Daniel G. Mazzone , Kim Lefmann

Due to the instrument's non-trivial resolution function, measurements on triple-axis spectrometers require extra care from the experimenter in order to obtain optimal results and to avoid unwanted spurious artefacts. We present a free and…

Instrumentation and Detectors · Physics 2021-02-17 T. Weber , R. Georgii , P. Böni

This paper presents a Matlab toolbox to perform basic image processing and visualization tasks, particularly designed for medical image processing. The functionalities available are similar to basic functions found in other non-Matlab…

Mathematical Software · Computer Science 2021-04-13 Alberto Gomez

Single molecule F\"orster resonance energy transfer (smFRET) is a powerful experimental technique for studying the properties of individual biological molecules in solution. However, as adoption of smFRET techniques becomes more widespread,…

Computational Engineering, Finance, and Science · Computer Science 2014-12-22 Rebecca R. Murphy , Sophie E. Jackson , David Klenerman

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

Computer Vision and Pattern Recognition · Computer Science 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

FcsIT is a platform-independent, open-source tool for calculating the correlation and fitting fluorescence correlation spectroscopy data. The software is written in Python and uses a powerful Dear PyGUI engine for its interface. It provides…

Quantitative Methods · Quantitative Biology 2026-04-01 Tomasz Kalwarczyk

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

Biological Physics · Physics 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman

We present a new Matlab toolbox for generating phase and amplitude patterns for digital micro-mirror device (DMD) and liquid crystal (LC) based spatial light modulators (SLMs). This toolbox consists of a collection of algorithms commonly…

Instrumentation and Detectors · Physics 2020-02-26 Isaac C D Lenton , Alexander B Stilgoe , Timo A Nieminen , Halina Rubinsztein-Dunlop
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