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Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution is limited by the fluorescence lifetime to nanoseconds at most. At the ensemble level, two-dimensional spectroscopy…

Optics · Physics 2024-10-11 Sanchayeeta Jana , Simon Durst , Markus Lippitz

Summary: Biospectrogam is an open-source software for the spectral analysis of DNA and protein sequences. The software can fetch (from NCBI server), import and manage biological data. One can analyze the data using Digital Signal Processing…

Quantitative Methods · Quantitative Biology 2012-10-05 Naman Turakhia , Nilay Chheda , Manish K. Gupta , Ruchin Shah , Jigar Raisinghani

The program package SME (Spectroscopy Made Easy), designed to perform an analysis of stellar spectra using spectral fitting techniques, was updated due to adding new functions (isotopic and hyperfine splittins) in VALD and including grids…

Instrumentation and Methods for Astrophysics · Physics 2017-10-31 N. Piskunov , T. Ryabchikova , Yu. Pakhomov , T. Sitnova , S. Alexeeva , L. Mashonkina , T. Nordlander

Several techniques have been employed for the direct visualization of cytoskeletal filaments and their associated proteins. Total-internal-reflection-fluorescence (TIRF) microscopy has a high signal-to-background ratio, but it suffers from…

Biological Physics · Physics 2022-01-21 Yazgan Tuna , Amer Al-Hiyasat , Jonathon Howard

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

We here present SIMLR (Single-cell Interpretation via Multi-kernel LeaRning), an open-source tool that implements a novel framework to learn a sample-to-sample similarity measure from expression data observed for heterogenous samples. SIMLR…

Genomics · Quantitative Biology 2018-01-22 Bo Wang , Daniele Ramazzotti , Luca De Sano , Junjie Zhu , Emma Pierson , Serafim Batzoglou

Providing high-quality electron images and hyperspectral X-ray maps is a focus of many modern electron microscopy laboratories. Nevertheless, further image processing and annotations are often needed to prepare them for publications and…

Image and Video Processing · Electrical Eng. & Systems 2021-03-24 Noah Kraft , Anette von der Handt

GAMMA_FLOW is an open-source Python package for real-time analysis of spectral data. It supports classification, denoising, decomposition, and outlier detection of both single- and multi-component spectra. Instead of relying on large,…

Machine Learning · Computer Science 2025-11-13 Viola Rädle , Tilman Hartwig , Benjamin Oesen , Emily Alice Kröger , Julius Vogt , Eike Gericke , Martin Baron

As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routine light microcopy. At the single-molecule level, analysis…

Biomolecules · Quantitative Biology 2021-07-07 Jack W Shepherd , Ed J Higgins , Adam J M Wollman , Mark C Leake

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

Simulations with high accuracy are an essential part of scientific research to accelerate the innovation process. They are especially useful for finding novel approaches or optimizing existing methods. Today, powerful software tools are…

Medical Physics · Physics 2022-08-31 Patrick Vogel , Martin A. Rückert , Thomas Kampf , Volker C. Behr

Deep learning holds immense promise for spectroscopy, yet research and evaluation in this emerging field often lack standardized formulations. To address this issue, we introduce SpectrumLab, a pioneering unified platform designed to…

Scalable atom-based quantum platforms for simulation, computing, and metrology require fast high-fidelity, low-loss imaging of individual atoms. Standard fluorescence detection methods rely on continuous cooling, limiting the detection…

Spectral line observations are an indispensable tool to remotely probe the physical and chemical conditions throughout the universe. Modelling their behaviour is a computational challenge that requires dedicated software. In this paper, we…

Instrumentation and Methods for Astrophysics · Physics 2024-11-06 Thomas Ceulemans , Frederik De Ceuster , Leen Decin , Jeremy Yates

We describe a new software package for simulating channelised, high-time resolution data streams from radio telescopes. The software simulates data from the telescope and observing system taking into account the observation strategy,…

Instrumentation and Methods for Astrophysics · Physics 2022-05-09 Rui Luo , George Hobbs , Suk Yee Yong , Andrew Zic , Lawrence Toomey , Shi Dai , Alex Dunning , Di Li , Tommy Marshman , Chen Wang , Pei Wang , Shuangqiang Wang , Songbo Zhang

SMeagol is a software tool to simulate highly realistic microscopy data based on spatial systems biology models, in order to facilitate development, validation, and optimization of advanced analysis methods for live cell single molecule…

Biological Physics · Physics 2017-05-08 Martin Lindén , Vladimir Ćurić , Alexis Boucharin , David Fange , Johan Elf

Multiview light sheet fluorescence microscopy (LSFM) allows to image developing organisms in 3D at unprecedented temporal resolution over long periods of time. The resulting massive amounts of raw image data requires extensive processing…

Quantitative Methods · Quantitative Biology 2015-08-12 Christopher Schmied , Peter Steinbach , Tobias Pietzsch , Stephan Preibisch , Pavel Tomancak

Spectroscopy is the most important method for probing the structure of molecules. However, predicting molecular spectra on classical computers is computationally expensive, with the most accurate methods having a cost that grows…

Quantum Physics · Physics 2025-10-06 Liam P. Flew , Ivan Kassal

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

In fluorescence microscopy, spectral unmixing aims to recover individual fluorophore concentrations from spectral images that capture mixed fluorophore emissions. Since classical methods operate pixel-wise and rely on least-squares fitting,…

Computer Vision and Pattern Recognition · Computer Science 2026-03-26 Federico Carrara , Talley Lambert , Mehdi Seifi , Florian Jug