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Single nanoparticle tracking using optical microscopy is a powerful technique with many applications in biology, chemistry and material sciences. Despite significant advances, localising objects with nanometric position accuracy in a…

The ability to spatially and temporally map nanoscale environments in situ over extended timescales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins,…

Optics · Physics 2023-10-04 Guangjie Cui , Do Young Kim , Di Zu , Guanbo Chai , Somin Eunice Lee

Watching the motion of electrons on their natural nanometre length- and femtosecond time scales is a fundamental goal and an open challenge of contemporary ultrafast science. Optical techniques and electron microscopy currently mostly…

Optics · Physics 2018-01-23 Jan Vogelsang , Germann Hergert , Dong Wang , Petra Groß , Christoph Lienau

We introduce a novel approach for all-optical trapping and manipulation of absorbing aerosol particles based on a photophoretic force. We demonstrate experimentally, in open air, the robust three-dimensional guiding of agglomerates of…

Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…

Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles in a medium of known viscosity. However, these assumptions are typically not fulfilled…

Accurately measuring the size, morphology, and structure of nanoparticles is very important, because they are strongly dependent on their properties for many applications. In this paper, we present a deep-learning based method for…

Materials Science · Physics 2022-07-29 Claudius Zelenka , Marius Kamp , Kolja Strohm , Akram Kadoura , Jacob Johny , Reinhard Koch , Lorenz Kienle

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40…

Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterisation of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background…

Achieving high-throughput, comprehensive analysis of single nanoparticles to determine their size, shape, and composition is essential for understanding particle heterogeneity with applications ranging from drug delivery to environmental…

In cold atomic systems, fast and high-resolution microscopy of individual atoms is crucial, since it can provide direct information on the dynamics and correlations of the system. Here, we demonstrate nanosecond-scale two-dimensional…

Due to its rising importance in science and technology in recent years, particle tracking in videos presents itself as a tool for successfully acquiring new knowledge in the field of life sciences and physics. Accordingly, different…

Data Analysis, Statistics and Probability · Physics 2021-02-01 Kristina Dingel , Rico Huhnstock , André Knie , Arno Ehresmann , Bernhard Sick

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

Confocal microscopy in combination with real-space particle tracking has proven to be a powerful tool in scientific fields such as soft matter physics, materials science and cell biology. However, 3D tracking of anisotropic particles in…

Soft Condensed Matter · Physics 2015-05-20 T. H. Besseling , M. Hermes , A. Kuijk , B. de Nijs , T. -S. Deng , M. Dijkstra , A. Imhof , A. van Blaaderen

We experimentally demonstrate a non-imaging approach to displacement measurement for complex scattering materials. By spatially controlling the wave front of the light that incidents on the material we concentrate the scattered light in a…

Optics · Physics 2015-06-03 E. G. van Putten , A. Lagendijk , A. P. Mosk

The investigation of liquid flow at the nanoscale is a key area of applied research with high relevance to Physics, Chemistry and Biology. We introduce a method and a device that allows to spatially resolve liquid flow by integrating an…

Mesoscale and Nanoscale Physics · Physics 2016-08-24 R. F. Neumann , M. Engel , M. Steiner

An innovative method based on the traversal of rays, originating from detected particles, through a three-dimensional grid of voxels is presented. The methodology has as main advantage that the outcome of the method is independent of the…

Fluid Dynamics · Physics 2020-08-07 Mickael Bourgoin , Sander Huisman

We present a versatile setup for investigating the nanofluidic behavior of nanoparticles as a function of the gap distance between two confining surfaces. The setup is designed as an open system which operates with small amounts of…

Soft Condensed Matter · Physics 2017-01-17 Stefan Fringes , Felix Holzner , Armin W. Knoll

In this study we present an interferometric technique based on multiple wavelengths to capture the transient free surface contour of nanoliter drops spreading on a wettable surface, in particular close to the three-phase contact line.…

Fluid Dynamics · Physics 2024-12-24 Timo Richter , Mathis Fricke , Peter Stephan , Cameron Tropea , Jeanette Hussong

Anti-Brownian traps confine single particles in free solution by closed-loop feedback forces that directly counteract Brownian motion. The extended-duration measurement of trapped objects allows detailed characterization of photophysical…

Instrumentation and Detectors · Physics 2023-05-15 Allison H. Squires , Abhijit A. Lavania , Peter D. Dahlberg , W. E. Moerner