Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy
Abstract
This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments.
Keywords
Cite
@article{arxiv.0911.5040,
title = {Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy},
author = {Nilanthi Warnasooriya and Fadwa Joud and Philippe Bun and Gilles Tessier and Maite Coppey-Moisan and Pierre Desbiolles and Michael Atlan and Marie Abboud and Michel Gross},
journal= {arXiv preprint arXiv:0911.5040},
year = {2015}
}