Related papers: Light sheet and light field microscopy based on sc…
Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…
Feature detectors and descriptors are key low-level vision tools that many higher-level tasks build on. Unfortunately these fail in the presence of challenging light transport effects including partial occlusion, low contrast, and…
Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…
Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…
We develop a novel hyperspectral imaging system using structured illumination in an SLM-based Michelson interferometer. In our design, we use a reflective SLM as a mirror in one of the arms of a Michelson interferometer, and scan the…
Light field (LF) imaging captures both angular and spatial light distributions, enabling advanced photographic techniques. However, micro-lens array (MLA)- based cameras face a spatial-angular resolution tradeoff due to a single shared…
Light-sheet fluorescence microscopy (LSFM) is a cutting-edge volumetric imaging technique that allows for three-dimensional imaging of mesoscopic samples with decoupled illumination and detection paths. Although the selective excitation…
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…
Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…
Rapidly developing augmented reality (AR) and 3D holographic display technologies require spatial light modulators (SLM) with high resolution and viewing angle to be able to satisfy increasing customer demands. Currently available SLMs, as…
Compact, lightweight and high-performance spatial light modulators (SLMs) are crucial for modern optical technologies. The drive for pixel miniaturization, necessary to improve their performance, has led to a promising alternative, active…
Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…
Accurate calibration of internal parameters is a crucial yet challenging prerequisite for 3D reconstruction using light field cameras. In this paper, we propose a linear fractional transformation(LFT) parameter $\alpha$ to decoupled the…
Fiber-based confocal endomicroscopy has shown great promise for minimally-invasive deep-tissue imaging. Despite its advantages, confocal fiber-bundle endoscopy inherently suffers from undersampling due to the spacing between fiber cores,…
Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…
Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…
Over the last two decades, spatial light modulators (SLMs) have revolutionised our ability to shape optical fields. They grant independent dynamic control over thousands of degrees-of-freedom within a single light beam. In this work we test…
Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…
Most living organisms show highly conserved physiological changes following a 24-hour cycle which goes by the name of circadian rhythm. Among experimental models, the effects of light-dark cycle have been recently investigated in the larval…