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Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus…

Optics · Physics 2022-02-01 Gargi Sharma , Kanwarpal Singh

Multimode fibers (MMFs) are attractive ultra-thin replacements for state-of-the-art endoscopes, but the phase randomization in propagation through MMFs poses a major hurdle for imaging and focusing of light. Recently, this challenge has…

Optics · Physics 2015-06-30 Shamir Rosen , Doron Gilboa , Ori Katz , Yaron Silberberg

Mask-based lensless imagers use simple optics and computational reconstruction to design compact form factor cameras with compressive imaging ability. However, these imagers generally suffer from poor reconstruction quality. Here, we…

For decades since the development of phase-contrast optical microscopy, an analogous approach has been sought for maximizing the image contrast of weakly-scattering objects in transmission electron microscopy (TEM). The recent development…

The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Ganna Platonova , Dalibor Stys , Pavel Soucek , Kirill Lonhus , Jan Valenta , Renata Rychtarikova

A novel phase retrieval algorithm for broadband hyperspectral phase imaging from noisy intensity observations is proposed. It utilizes advantages of the Fourier Transform spectroscopy in the self-referencing optical setup and provides,…

Image and Video Processing · Electrical Eng. & Systems 2020-06-03 Igor Shevkunov , Vladimir Katkovnik , Karen Egiazarian

Cardiac contraction is a rapid, coordinated process that unfolds across three-dimensional tissue on millisecond timescales. Traditional optical imaging is often inadequate for capturing dynamic cellular structure in the beating heart…

Computer Vision and Pattern Recognition · Computer Science 2025-11-06 Yi Gong , Xinyuan Zhang , Jichen Chai , Yichen Ding , Yifei Lou

Fluorescence microscopy (FM) imaging is a fundamental technique for observing live cell division, one of the most essential processes in the cycle of life and death. Observing 3D live cells requires scanning through the cell volume while…

Image and Video Processing · Electrical Eng. & Systems 2026-01-28 Chenwei Wang , Zhaoke Huang , Zelin Li , Wenqi Zhu

Continuous wavefront sensing benefits space observatories in on-orbit optical performance maintenance. To measure the phase of a wavefront, phase retrieval is an attractive technique as it uses multiple point spread function (PSF) images…

Instrumentation and Methods for Astrophysics · Physics 2024-09-17 Hyukmo Kang , Kyle Van Gorkom , Meghdoot Biswas , Daewook Kim , Ewan S. Douglas

A new method for dark field imaging is introduced which uses scanned electron diffraction (or 4DSTEM - 4-dimensional scanning transmission electron microscopy) datasets as its input. Instead of working on simple summation of intensity, it…

Materials Science · Physics 2024-09-18 Ian MacLaren , Andrew T. Fraser , Matthew R. Lipsett , Colin Ophus

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

Dark-field X-ray microscopy (DFXM) is a nondestructive full-field imaging technique providing three dimensional mapping of microstructure and local strain fields in deeply embedded crystalline elements. This is achieved by placing an…

Temporal focusing two-photon microscopy enables high resolution imaging of fine structures in vivo over a large volume. A limitation of temporal focusing is that signal-to-background ratio and resolution degrade rapidly with increasing…

Ultrafast X-ray diffraction by the LCLS free-electron laser has been used to probe Charge Density Wave (CDW) systems under applied external currents. At sufficiently low currents, CDW wavefronts bend in the direction transverse to the…

We present a tomographic cosmological weak lensing analysis of the HST COSMOS Survey. Applying our lensing-optimized data reduction, principal component interpolation for the ACS PSF, and improved modelling of charge-transfer inefficiency,…

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…

Signal Processing · Electrical Eng. & Systems 2023-07-18 Olivier Leblanc , Matthias Hofer , Siddharth Sivankutty , Hervé Rigneault , Laurent Jacques

Mask-based lensless cameras offer a novel design for imaging systems by replacing the lens in a conventional camera with a layer of coded mask. Each pixel of the lensless camera encodes the information of the entire 3D scene. Existing…

Image and Video Processing · Electrical Eng. & Systems 2022-12-23 Yucheng Zheng , M. Salman Asif

We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…

Quantitative Methods · Quantitative Biology 2009-11-10 R. V. Krishnan , H. Saitoh , H. Terada , V. E. Centonze , B. Herman

We report the creation of Ge$_2$Sb$_2$Te$_5$ metasurfaces on sapphire substrates by the ablation method and the study of their structural properties by scanning electron microscopy (SEM), atomic force microscopy (AFM), and optical…

Applied Physics · Physics 2020-12-03 D. V. Bochek , D. A. Yavsin , A. B. Pevtsov , K. B. Samusev , M. F. Limonov
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